胚胎来源的猪多能性干细胞系建立及其多能性改善研究-生物学；发育生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ． ． ． ． ． ． ．I Abstract in English ． ． ． ．．III 1 Introduction ． ．．1 1．1 Summary ofpluripotent stem cells． · ·1 1．1．1 The definition and classification of pluripotent stem cells ．1 1．1．2 Embryonic stem cell research in other species 2 1．2 The research value and application prospect of porcine embryonic stem cells ．3 1．2．1 Summary ．．3 1．2．2 Influence factor ．4 1．3 The regulation pathways ofpluripotent stem cell ． ．．5 1．3．1 The state ofpluripotent stem cell ．．7 1．3．2 The signalling pathway ··9 1．3．3 The particularity ofpluripotency and signalling pathways in pigs ．．10 1．4 The appraisal standard and the present situation ．．10 1．5 Chemically defined and selum．free medium ．．1 1 1．6 The purposes of the research ．1 1 2 Materials and methods ． ． ． 。。13 2．1 Materials ．1 3 2．1．1 Animals and materials ．．13 2．1．2 Equipments 13 2．1．3 Reagents and kits ． ．．14 2．1．4 Commen reagentpreparation 15 2．2 Methods ． ． 16 2．2．1 IVF embryo culture ． ．． ． ．16 2．2．2 Cell culture 1 7 2．2．3 Packaging lentivirus ．1 8 2．2．4 Pluripotent characterization ．．19 2．2．5 Gene editing ． 21 2．2．6 Somatic Cell Nuclear Transfer and the development statistics ．．22 2．2．7 Chimera preparation ．22 2．2．8 Chimera validation ． ．．22 2．2．9 Statistical analysis ． 22 3 Results and analysis ． 24 3．1 Generation of porcine pluripotent stem cell line from IVF blastocysts ．．24 3．1．1 The effects of oxygen concentration the establishment of pPSCs ．24 3．1．2 The effects of media the establishment of pPSCs ．24 万方数据 东北农业大学理学博士学位论文3 东北农业大学理学博士学位论文 3．1．3 The effects of embryonic age the establishment of pPSCs 25 3．1．4 Generation ofa porcine pluripotent stem cell line from IVF blastocysts ．26 3．2 Characteriza