分子荧光法分别测定4种野生可食植物中的色氨酸和酪氨酸-食品科学.PDF

※分析检测 食品科学 2012, Vol.33, No.22 231 4 1 1 2 2 高向阳 ，张凯歌 ，孔欣欣 ，游新侠 (1.河南农业大学食品科学技术学院，河南 郑州 450002 ；2.郑州科技学院食品科学系，河南 郑州 450064) 摘 要：为建立分别测定食品中酪氨酸和色氨酸的新方法，选择郑州地区的贼小豆、续断菊、车前草、火棘果野生 植物为样品，以6mol/L的氢氧化钠溶液水解，用分子荧光法进行测定。结果表明：在不同的酸度条件下，样品中色 氨酸和酪氨酸测定的质量分数均值分别为1.37～2.63mg/g和7.98～25.83mg/g 。相对标准偏差不大于5.4%，色氨酸在 实验浓度范围内线性良好，方法检出限为0.011μg/mL、定量限为0.037μg/mL，回收率为93.5%～105%；酪氨酸在实 验浓度范围内线性良好，方法检出限为0.033μg/mL，定量限为0.11μg/mL，回收率为94.6%～100.5%。该方法操作简 便、灵敏、快速，可为食品新资源的开发提供科学参考，有一定的应用价值。 分子荧光法；色氨酸；酪氨酸；续断菊；车前草；贼小豆；火棘果 Determination of Tryptophan and Tyrosine in Four Edible Wild Plants by Molecular Fluorescence Spectrometry 1 1 2 2 GAO Xiang-yang ，ZHANG Kai-ge ，KONG Xin-xin ，YOU Xin-xia (1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China； 2. Department of Food Science, Zhengzhou Institute of Science and Technology, Zhengzhou 450064, China) Abstract ：A new method was established to determine tryptophan and tyrosine in four edible plants wildly grown in Zhengzhou region, Sonchus asper, Plantago asiatica, Vigna minima and Pyracantha fortuneana . Samples were hydrolyzed with with 6 mol/L sodium hydroxide solution determined by molecular fl uorescence spectrometry. The average contents of tryptophan and tyrosine in the wild plants were 1.37–2.63 mg/g and 7.98–25.83 mg/g, respectively, with a relative standard deviation equal to or less than 5.4% (n = 5). The proposed tryptophan standard curve showed good linear relationship over the concentration range of 0.00–0.10 μg/mL. The method exhibited