若干茎叶除草剂对植物叶绿素荧光的影响-农药学专业论文.docxVIP

AbstractAbstract Abstract Abstract Herbicides bioassay is a method to identify the activity of herbicide by weed’S responses．It is important in herbicide development,production and application． Because of longer period and more severity condition，it has limited herbicide research strictly． In this research，we eombited the rapid and convenient Chlorophyll Fluorescence technology and normal bioassay．Five common herbicide(Bromoxynil，Metribuzin, Glyphosate，Fluroxypyr and 2,4·D)was used to indoor pot experiment,and the Chlorophyll fluorescence parameter change of the Echinochloa crusgalli亿．)Beauv, Abutilon theophrasti Medicus,wheat，maize and sogoearl caused by the herbicide has been researched,Wet weight and dry weight effective WaS calculated also．Through the above experiments we conclude that： No parameter Can fit all the herbicides，SO we should classify． 1．The method is applicable to photosynthesis inhabitor(Bromoxynil and Metribuzin) and glyphosate．The chlorophyll fluorescence parameter RC／CSm is universal in the research of photosynthesis inhabitor．when the inhabition approximate 50％一70％is equal to 50％inhabition of biomass．We can’t discovery a common parameter within glyphosate，however the sensitivity of these parameters are also as strong as Bromoxynil and Metribuzin． 2．Fluroxypyr and 2，4-D ale auxin herbicides．The chlorophyll fluorescence parameters are stable in this research SO that the method can’t be utilized．It is necessary to go deep into the new leaf parameter． This study showed that the chlorophyll fluorescence is applicable in herbicide bioassay,Both photosynthesis inhabitor and glyphosate are available．This method is rapid and sensitive，and it is valuable to research further． Key Words：Herbicide Bioassay Chlorophyll Fluorescence —II— 绪论1绪论 绪论 1绪论 除草剂生物测定是利用生物体对除草剂的反应，来测定除草剂毒性及效果的 基本方法。除草剂的抑制程度以及除草剂的活性通过供试植物的生长量来确定， 通常以鲜重、干重和株高等生物量作为指标，但某些除草剂对植物的典型症状也 可用于定性定量测定11]-14]。 植株内部的各种新陈代谢作用是植物的生长发育所不可或缺的，这些代谢包 括光合作用(将光能转化为化学能)、呼吸作用(降解糖类为植物提供能量)、脂 类的合成、氨基酸及蛋白质