以聚苯乙烯-二乙烯苯为载体的多目标免疫亲和柱的制备及-食品科学.PDF

172 2016, Vol.37, No.20 食品科学 ※安全检测 以聚苯乙烯-二乙烯苯为载体的多目标免疫亲和柱的 制备及应用 * 张家宁，丁 轲 ，韩 涛，陈湘宁 （北京农学院食品科学与工程学院，食品质量与安全北京实验室，农产品有害微生物及农残安全检测与控制 北京市重点实验室，北京 102206 ） 摘  要： 3 - 选取 种不同聚苯乙烯 二乙烯苯基材，通过硝基化、氨基化、活化、偶联抗体等步骤制备免疫亲和柱， 用于4 T-2 HT-2 3 种镰刀菌毒素（玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇、 毒素、 毒素）的同时纯化，并将 种基材 Cleanert PS - 制备的免疫亲和柱进行比较。以 型 聚苯乙烯 二乙烯苯为基材制备的免疫亲和柱与抗体偶联的偶联率 为97.64%，且其空白柱对样品的吸附作用最小，3 个加标水平下的回收率为66.16%～112.80%，相对标准偏差为 3.09%～12.25%，可应用于实际样品的纯化。 - 关键词：聚苯乙烯二乙烯苯；免疫亲和柱；镰刀菌毒素 Preparation and Application of Multi-Target Immunoaffinity Columns with Polystyrene-Divinylbenzene (PS-DVB) as Carrier * ZHANG Jianing, DING Ke , HAN Tao, CHEN Xiangning (Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide, Beijing Laboratory of Food Quality and Safety, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China) Abstract: Three kinds of polystyrene-divinylbenzene (PS-DVB) resins were selected to prepare immunoaffinity columns (IAC) through nitration, amination, activation, and antibody coupling for the simultaneous purification of four Fusarium toxins, zearalenone, deoxynivalenol, T-2 and HT-2 toxins. Among these, the coupling ratio of Cleanert PS-type PS-DVB IAC was 97.64% and the absorption of its blank column for samples was