株乳酸芽子包杆菌的筛选初步鉴定及应用试验.pdf

第 31 卷第2 期 华南农业大学学报 Vo l. 31 , No.2 2010 年4 月 Journal of South China Agricultural University Ap r. 2010 3 株乳酸芽子包杆菌的筛选、初步鉴定及应用试验 徐春厚，潘俊福，相菲，韩伟，谢为天 (广东海洋大学动物医学系，广东湛江 524088) 摘要:通过对41 株产酸芽于包杆菌 Bαcillus 培养物的 HPLC 分析及抑菌活性和芽抱形成检测，筛选出 3 株能产生乳 酸、有抑菌活性、易形成芽抱的乳酸芽抱杆菌.通过形态染色特性观察、Biolog 系统鉴定及 16S rDNA 碱基序列分析， 初步鉴定3 株菌为芽抱杆菌SC Bacillus sp. SC 、芽抱杆菌 TR B. sp. TR 和蜡状芽抱杆菌 S11 B. ce陀ωstrain 511. 动 物饲养试验显示，乳酸芽抱杆菌益生素能提高兰黄鸡血清钙、磷、总蛋白、球蛋白、溶菌酶、IgG 、 IgA 含量及脾脏、胸 腺和法氏囊指数:饲料中添加5 glkg 的乳酸芽抱杆菌益生素，可使56 日龄三黄鸡的平均日增质量提高 13.61% ，料 质量比降低 18.37%. 关键词:乳酸芽抱杆菌;筛选;鉴定;应用试验;三黄鸡 中图分类号:TQ920. 6; Q939. 9 文献标识码:A 文章编号: 1001-411 X( 2010 )02-0117-04 Screening ,Preliminary Identification and Application Test of Three Bacillus Strains Producing Lactic Acid XU Chun-hou , PAN Jun-fu , XIANG Fei , HAN Wei , XIE Wei-tian (Department of Animal Medicine ,Guangdong Ocean University ,Zhanjiang 524088 ,China) Abstract :The culture of 41 Bαcillus strains producing acid was analysed by detection of HPLC , bacterio- static activity and sporulation. Three lactic acid Bαcillus strains producing lactic acid , antibacterial sub- stance and spore were identified. According to the morphological characteristics , staining property , Biolog systematic identification and 16S rDNA sequence analysis ,three strains were identified as Bαcillus sp. SC , B. sp. TR and B. cereus strain SLl. Animal feeding trials showed that lactic acid Bacillus probiotics could in- crease content of calcium ,phosphorus ,total protein ,globulin ,lysozyme ,lgG ,lgA in serum of three buff chick- ens ,and idexes of spleen ,thymus ,bursa of three buff chickens. To 56 day-old thre