干旱胁迫对春大豆超氧化物歧化酶活性和丙二醛含量的-中国农学通报.PDF

中国农学通报 2016,32(15):93-97 Chinese Agricultural Science Bulletin 干旱胁迫对春大豆超氧化物歧化酶活性和 丙二醛含量的影响 李雪凝，董守坤，刘丽君，徐亚会，高鑫宇，王利彬 （东北农业大学农学院，哈尔滨150030） 摘 要：大豆是一种对水分敏感的豆类作物，明确干旱对大豆叶片主要生理特性的影响，对于进行更好 的水分管理具有重要的理论和实践意义。笔者选用耐旱型品种‘黑农44 ’和敏感型品种‘黑农65 ’为材 料，测定了不同生育时期干旱及复水后大豆叶片SOD 活性和MDA 含量。结果表明：在干旱条件下， SOD 活性随干旱程度增加呈先增加后降低的趋势，活性增加幅度花期＞苗期＞鼓粒期，且‘黑农44 ’的 SOD 活性明显大于‘黑农65 ’；MDA 含量随干旱程度增加呈逐渐上升趋势，苗期＞开花期＞鼓粒期，且 ‘黑农44 ’明显小于‘黑农65 ’。复水后，SOD 活性和MDA 含量均逐渐降低；苗期干旱复水后MDA 含量 恢复较快，开花期干旱复水后恢复时间较长，鼓粒期干旱复水后不能恢复。 关键词：大豆；活性氧；水分；SOD；MDA 中图分类号：S513 文献标志码：A 论文编号：cas EffectofDroughtStressonSODActivityandMDAContentofSpringSoybean LiXuening,DongShoukun,LiuLijun,XuYahui,GaoXinyu,WangLibin (AgriculturalCollege,NortheastAgriculturalUniversity,Harbin150030) Abstract:Soybeanisoneofthemostsensitivecroptowaterinlegumes,toclarifytheeffectofdroughtstress onleafphysiologicalcharacteristicsofsoybeanhastheoreticalandpracticalimportancetowatermanagement. Drought-resistantvariety ‘Heinong 44’andsensitivevariety ‘Heinong 65’wereusedastestmaterialsand leafSODactivityandMDAcontentatdifferentgrowthstagesunderdroughtstressandafterre-wateringwere measured.TheresultsshowedthatSODactivityincreasedandthendecreasedwiththeincreaseofthedrought degree,theincreasingrangewasasfollows:floweringstage＞seedlingstage＞fillingstage,SODactivityof ‘Heionng44’wassignificantlystrongerthanthatof ‘Heinong65’.MDAcontentshowedanincreasingtrend withtheincreaseofthedroughtdegree,theincreasingtrendwasasfollows:seedlingstage＞floweringstage＞ fillingstage,andtheMDAcontentof ‘Heinong 44’wasobviouslylowerthanthatof ‘Heinong 65’.SOD activityandMDAcontentgraduallydecreasedafterre-watering;MDAcontentrecoveredfastinseedingstage, whiletherecoverytimeinf