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伊犁马dmrt3 mstn但 actn3基因多态性与运动性能的相关性分析
Correlation
Correlation Analysis of Polymorphisms of DMR T3,MSTN and
A CTN3 Genes with Spots Perfomance in Yili Horse Abtract
In this study.Yili horses were used for the main experiment object.D.^组乃,』淄7Ⅳand ACTN3 gene were chosen main candidate genes.Genetic polymorphisms were detected by PCR-RFLP.PCR-SSCP and DNA sequencing technique.Selected the gene of racing performance in Yili horses,found the association with
DMRT3.MslW and ACTN3 genes the sports performance.Provide basis fot preliminary studies and technica、
support for further exploration ofthe genetic mechanism of sports performance Yili horse breeds from the molecular level.The results were as follows:
1.Correlation analysis DMRT3 genetic polymorphism with gait traits in Yili horse
Genetic polymorphism detection 1 ofJDjI很T3 gene in different types ofYili horse.where D懈乃 gene polymorphismin in Yili horse was the first time to report.ne study found that the gene polymorphism in DMR乃,sequencing result showed也at the SNP was gCA.It was mutation tllat created
premature stop codon that results in truncated protein.There were three genotypes:CC,CA and AA,C was the dominant allele.After association analysis,Speed ofpacing type was faster than trotting type(PO.05).the SNP was not associate between D肘R乃gene mutation and gait type in Y诅i horse.but it had significant effect (尸0.05)ofspeed in Yili horse(Trotting type)and may be important site gait traits ofYili horse.,
2.Correlation analysis MSTN genetic polymorphism with the speed ofdifferent distance in Yili horse
Genetic polymorphism detection intron 1 ofMSTN gene in 4 groups ofYili horse.The study found that the gene polymorphism in MSTN,sequencing result showed that the SNP was g.66493 737 CT.There were three genotypes:CC,CT and TT,C was the dominant allele in 2 km and 1 5 km group,while T was the dominant allele in 3 km and 5 km group.After association analysis,in 5km group,this mutation had significant effect(P
O.05、of speed in Yili horse and may be im
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