海南近海石油降解菌的筛选、鉴定及降解性能.PDF

中国农学通报 2017,33(17):82-86 Chinese Agricultural Science Bulletin 海南近海石油降解菌的筛选、鉴定及降解性能 文少白，牛宗亮，季玉祥，唐天乐 （海南医学院热带医学与检验医学院，海口571199） 摘 要：为筛选出适应海南海洋环境的高效石油降解菌，以柴油为唯一碳源，通过分离、初筛和复筛，从 石油长期污染的海南近海海水中分离出1株高效的石油降解菌株X 10，经16S rDNA 序列分析，该菌株 被鉴定为无色菌，其对柴油的降解率为69.39% 。同时，研究分析不同pH 值、温度、初始接种量、外加碳 源对菌株柴油降解能力的影响。结果表明，在试验条件下，菌株X 10在初始pH 6.0 时降解效果最好，且 该菌pH 值适应范围较广，偏酸或偏碱的环境下该菌对柴油的降解效果均较好；培养温度对菌株石油降 解率的影响较大，最佳温度为30℃，降解率达67.94% ；最佳接种量为5% ；添加外加碳源葡萄糖、乳糖、甘 露醇对柴油降解率均有提高，但3 种外加碳源间差异不显著。 关键词：降解率；菌株；筛选 中图分类号：X55 文献标志码：A 论文编号：cas OffshorePetroleumDegradingBacteria:Screening,IdentificationandDegradationProperty inHainan WenShaobai,NiuZongliang,JiYuxiang,TangTianle (InstituteofTropicalMedicineandInspectionDivision,HainanMedicalUniversity,Haikou571199) Abstract:Thestudyaimstoscreenefficientpetroleumdegradingbacteriaadaptingtomarineenvironmentin Hainan.Takingdieselastheonlycarbonsource,thepetroleumdegradingstrainX10wasseparatedbyprimary andsecondaryscreeningfromthepetroleum-contaminatedcoastalareaofHainan, 16SrDNAsequencing showed that the strain was identified as Achromatium, and the degradation rate to diesel was 69.39%. Meanwhile,westudiedtheeffectsofpHvalue,culturetemperature,initialinoculationamountandexternal carbonsourceonthedegradationabilityofdiesel.Theresultsshowedthat:strainX10hadthebestdegradation effectattheinitialpH 6.0 undertheexperimentalconditions;thestraincouldadapttoawiderangeofpH value;underacidoralkalienvironment,thedegradationeffectofbacteriaondieselwasgood;theculture temperaturehadasignificantinfluenceonthepetroleumdegradationrate;thedegradationratewas 67.94%at 30℃,whichwastheoptimumtemperature;theoptimuminoculationamountwas 5%;thedegradationrateof diesel