鹅掌楸抗氧化物质提取及其特性的研究.PDF

中国农学通报 2012,28(31):29-35 Chinese Agricultural Science Bulletin 鹅掌楸抗氧化物质提取及其特性的研究 刘志祥，曾超珍 （中南林业科技大学生命科学与技术学院，长沙410004 ） 摘 要：为了研究鹅掌楸的抗氧化活性，为开发有应用前景的天然抗氧化剂奠定基础，以亚油酸过氧化抑 制率为参照，通过单因素试验和正交试验研究了鹅掌楸抗氧化物质的最优提取条件，以及pH 和热处理 对其抗氧化活性的影响，并探讨了鹅掌楸抗氧化物质对油脂抗氧化的作用。结果表明，鹅掌楸抗氧化物 质提取的最佳条件为：乙醇体积分数为75% ，料液比1:12，提取时间5 min ，微波功率210 W 。热处理对 其抗氧化能力的影响较大，pH 的影响不显著；鹅掌楸提取物对猪油的抗氧化作用明显，且随着鹅掌楸提 取物浓度的增大，其抑制猪油生成氢过氧化物的能力增强。VE 和柠檬酸对鹅掌楸提取物的抗氧化作用 有协同作用，协同增效的强弱顺序为：柠檬酸VE ，而VC 没有明显的协同增效作用。 关键词：鹅掌楸；提取工艺；抗氧化物质 中图分类号：TS201.3 文献标志码：A 论文编号：2012-2209 TheStudyonAntioxidantActivityandPropertiesofExtractsfromLiriodendronchinense Liu Zhixiang, Zeng Chaozhen (CollegeofLifeScienceandTechnology,CentralSouthUniversityofForestryandTechnology, Changsha 410004) Abstract:The objective of this study was to select the optimal microwave extracting technology of antioxidant from Liriodendron chinense, and develop an applicable antioxidant. With the antioxidantion of linoleic acid as a reference, the conditions of extracting antioxidant from Liriodendron chinense were studied through single factors experiments and orthogonal experiment. Effects of pH value and heat treatment on the antioxidant activity of extracts were investigated. The activity of extracts as an antioxidant in lard and vegetable oil was examined. The results showed that the optimal extracting conditions for the antioxidant of liriodendron chinense were as follows: ethanol 75% , solid-liquid radio 1:12, extraction time 5 min, microwave power 210 W. Heat treatment significantly influenced the antioxidant activity of extracts, but pH values did not. The antioxidantion of extracts was obvious in lard. With the concentration of the extract