荧光探针应用技.pptVIP

2007-11-16 2008-4-18 2008-8-26 2009-2-13 乳头瘤病毒感染引起的疣状表皮发育不良 乳头瘤病毒感染引起的疣状表皮发育不良 EVER2蛋白 细胞膜 HPV 在生命科学、材料科学等的研究中，荧光探针起重要作用。 在许多领域荧光探针已经取代放射性核素标记技术成为标准的研究工具，在检测速度和易用性方面都具有无可比拟的优点 随着激光扫描共聚焦显微镜在国内的引进和广泛应用，以及流式细胞仪和荧光显微镜在国内的普及，对各种荧光标记物的需求和了解日益迫切。 课程意义 荧光探针发展简史 荧光探针基本理论及选择应用 荧光探针负载技术 绿色荧光蛋白的发现及其应用 超敏检测技术 荧光探针在病原生物学领域的应用 激光扫描共聚焦显微镜技术简介及其荧光探针选择 分子信标的原理、量子点荧光探针等介绍 免疫荧光组织化学技术原理及应用 主要内容 * 光 * 荧光 * Mineral Fluorescence * 探针和荧光探针 * Sabrina Mattoli. Allergen-Induced Generation of Mediators in the Mucosa. Environmental Health Perspectives ? VOLUME 109 | SUPPLEMENT 4 | August 2001 * A little bit of science Most of you know that Im working in cell biology. The beauty in this field is to discover every day a new, little tiny piece of the secret of life - to understand how nature works… For most of you, my stuff is very abstract, I know! To prove you the contrary - science can be very artistic and beautiful - I would like to show you for example this picture. I took it this morning on an ordinary fluorescent microscope. 4月27日 A little bit of science Most of you know that Im working in cell biology. The beauty in this field is to discover every day a new, little tiny piece of the secret of life - to understand how nature works… For most of you, my stuff is very abstract, I know! To prove you the contrary - science can be very artistic and beautiful - I would like to show you for example this picture. I took it this morning on an ordinary fluorescent microscope. This picture illustrates the skeleton of skin cell. The skeleton of a cell - called cytoskeleton - is mainly composed of actin, which is labeled here with a green fluorescent probe. Actin is for the cell cytoskeleton what bones are for our skeleton, and thus, this molecule plays a major role in keeping the shape of a cell. Well, I dont have to mention that this extra-ordinary actin cytoskeleton resembles very much a spider web - the thickest filaments you can see are approximately 5000 times thinner than a human hair! O