低夜温下黄瓜雌性分化相关代谢与蛋白质变化的初步分析园艺学蔬菜学专业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ．I Abstract in English．．． ．．．．．．．． ．． ．．． ．．．．．． ． ．．．．．． -．． III 1 Introduction 1 1．1 Purpose and significance 1 1．2 National and abroad literature summarization 。1 1．2．1 Genetic basis of determination in Cucumber 1 1．2．2 The physiological and biochemical basis of differentiation in cucumber and the influence ofenvironment ．2 1．2．3 The transcriptional study of determination in Cucumber ． ． ．．．．．．．5 1．2．4 Advances in proteomics research ．． 6 1．3 Existing problems and prospects ．．．．．．．．．． ． ．．．．．．．．． ．．．．．．．．．． ．．．．．． ．．．-．．6 I．4 Research contents ．．．．．．．．．．．．．． ．． ．．．．．．．．．．．．． ．．．．．．．．．．．．． ．．．．．．．．．．．． ．．7 1．4．1 Main research contents ．．7 1．4．2 Technology routes ． ．．．．．．．．．．．． ． ．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．． ．．．．．．． ．7 2 Materials and methods 8 2．1 Experiment matedals 8 2．2 Plant cultivation and temperature treatments 8 2．3 Sample preparation．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．． ． ．．．．．．．．．．．．．．．．．．．． ． ．．．．．．．．．．．．． ．．8 2．4 Determination ofmetal)olism related indexes ．．8 2．4．1 Determination of sugars ．8 2．4．2 Determination ofhormones and amino acid ．．10 2．4．3 Determination ofrelated enzymes ．．1 1 2．5 Proteome difference analysis．．．．．．． ．． ．．．．．．．．．．．．．．．． ．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．12 2．5．1 Separation ofprotein samples．．．．．．．．． ． ．．．．．．． ．．．．．．．．．．．．． ． ． ．．．12 2．5．2 Determination ofprotein concentration by bradford．．．．．．．．．．．． ． ． ． ． ． ．．．．．．．．．．．．．．．1 3 2．5．3 2-DE two-dimensional gel electrophoresis．．．．．．．．．．．．．．．． ．．．． ． ．．．．．．．．．．．．．14 2．5．4 Gel dyeing．．．．．．．．．．． ． ．．．．．．．．．．．．．．．．．．．．． ． ．．． ．．．．．．．．．．．．．．．16 2．5．5 Gel scanning and image analysis．．．．．．．．．．．．．．．．．． ．．．． ． ． ．．． ．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．． 16 2．5．6 Mass spectrometry analysis．．．．．．．．．．．．．．．． ．．． ． ．． ． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．1 7 2．6 Verification ofprotein differential point in transcription level． ． ．．．． ． ．．． ．．．．．．．．．．．．．17 2．6．1 Extraction ofRNA ．17 2．6．2 Synthesis ofcDNA fi