基于线粒体RNAs序列分析雁形目家禽的分歧时间.PDFVIP

中国农学通报 2011,27(7):331-334 Chinese Agricultural Science Bulletin 基于线粒体RNAs序列分析雁形目家禽的分歧时间 郭 军，汤青萍，章双杰，邹剑敏 （中国农业科学院家禽研究所，江苏扬州225003 ） 摘 要：雁形目鸟类具有重要的社会经济意义和科学研究价值，部分种类已被驯化成为重要的家禽，然而 雁形目内部物种之间的关系仍存在许多争议。由于线粒体基因组具有独特的遗传特点，因此选用线粒 体RNAs 序列标定雁形目部分鸟类的分歧时间，旨在进一步查明该类群系统进化关系。首先，采用酚仿 法分别从太湖鹅和朗徳鹅血液中提取基因组DNA ，PCR 扩增获得线粒体RNAs 基因序列。然后，从 GenBank 中提取10种鸟类线粒体DNA 的同源序列，以鸵鸟为外群，采用贝叶斯法结合RNA 结构信息重 建系统发生关系；最后，以黑雁属与雁属的分歧时间为锚定点，利用r8s 软件分析了雁形目物种间的分歧 时间。结果表明，中国鹅与欧洲鹅的分歧时间为0.61 百万年，番鸭与家鸭的分歧时间为15.5 百万年。依 据家鹅祖先分歧时间，联系当时发生的地学事件，推测家鹅祖先物种分化与更新世冰川运动有关。研究 结果有助于中国水禽遗传资源的保护与利用。 关键词：雁形目；线粒体RNAs；分歧时间；物种形成 中图分类号：S835 文献标志码：A 论文编号：2010-3630 DivergenceTimeBetweenDomesticFowlsinAnseriformesInferredfromMitochondrialRNAs Guo Jun, Tang Qingping, Zhang Shuangjie, Zou Jianmin (InstituteofPoultryScience,CAAS,Yangzhou Jiangsu 225003) Abstract: Many water fowls have a long history of domestication for socio-economic reasons. However, phylogenetic relation in this group remains contradictory. In this study, the divergence time between water fowls has been inferred with mitochondrial RNAs ，which owned unique heredity mode ，in order to elucidate speciation events in domestic geese. Firstly, totle DNA has been extracted from Taihue goose and Landaise goose, which stand respectively for Chinese geese and European geese. Mitochondrial RNAs have been amplified by PCR method. Secondly, 10 complete mtDNA sequences have been picked up from GenBank. Then, phylogenetic tree has been reconstruct with Bayesian method and RNA secondary structure. The ostrich mtDNA has been assigned to outgroup. At last, the divergence time is inferred with r8s. The split time of Anser-Bra