cyp2a6基因多态性与烟草代谢物相关性冠心病的关系临床检验诊断学专业论文.docxVIP

硕士学位论文ABSTRACT 硕士学位论文 ABSTRACT OBJECTIVE：To explore four polymorphisms of CYP2A6(1，2，3 and 4)for association with tobacco metabolin related coronary heart disease；to explore a convenient method to optimize PCR conditions． METHODS：The genomic DNA was extracted from 205 patients 、衍tll essential coronary heart disease(CHD)and 208 health adult Han subj ects(NC)in Hunan．Based on their smoke habit，they were devided into smokers(SM)and nonsmokers groups(NS)，207 and 206 subject respectively．Association analysis was employed．Uniform Design was applicated to optimize the four factors that may affect the