《SNT1202-2003-食品中转基因植物成分定性PCR检测方法》.pdfVIP

#%S*SNB%B*ZtB PCR jJ!g :g Protocol of the qualitative polymerase chain reaction for detecting genetically modified plant components in food ~3n~~f+t:~)1m~kaa*k@m~1milfiljt%*h%m%~n m fl :.asrsssmxFa+le.mmi% H#@t~#~R~@)~#t5%~4BH;f.;4%~t%TmtBfiRB2P;Z~BB~~%3iWf% ABRf@!HBBAt+;9~%E*eRR+% f : ~ t g l 5 l M F f ] R ~ 1 ~ ~ ~ z b : ~ H ~ * ~ ~ . GB/T 6682 j)$i%SH7k%#j#IBtk(eqv IS0 3696: 1987) SNIT 1193 J#B@!%3Z*Bjk SNIT 1194 M @ B # ~ # I % ~ ~ @ B ! ! ~ # ~ # ~ ! # - ) ~ % S N / T 1195 hEi+$$SL!43)Z?4ICR UB!!YJ S N / T 1196 3 9 %PCR %%!!h? S N / T 1197 ?h%RRB33)2# PCR 3$ildhk S N / T 1199 # l % + R B 3 3 )PCR @I%- SN/T 1204 t$~B#%fi~SC~~%3~r3t3)TdU~%XPCR %%t%?f? TPl@%%HT*%* 3. 1 PCRIpolymerase chain reaction,m% PCR. 3.2 DNA 1 de~x~ribonucleicacid,%%@@j@. 3 . 3 dNTP:deoxyribonucleoside triphosphate,8#$t%3?@@. 3 . 4 dATPldeoxyadenosine tr~phosphate~Llk!%IlTT@@. 3 . 5 dCTP:deoxycytidine t r i p h ~ s p h a t e , a # S m B E - R @ ~ 3.6 dGTPIdeoxyguanosine t r i p h o s p h a t e , R # $ € ~ ~ 7 @ @ 3 . 7 dUTP deox~ur~dinetriphosphate, Jl$!$G$e3.aft@ 3.8 bp base pair, @SN. 3.9 Tag B r D N A J@*m. 3. 10 Irls, tris (hydroxymethyl) amlnoniethanc, !-:(%!pg),%V%$., 3. 11 T E : T ~ I ~ - C I . E D T A $ ~ ! ~ ~ ~ $ . . 3. 12 I.ectinl44l%~. 3.13 ZEINi#%B$#E$. 3.14 ~~3-~~~case1~hos~hoenil~~ruvate-carboxylahc,b#@~~~aj~@;)fat.(t:~. 3. 15 ~ a t a t ~ n , ~ # # Z E % Z ? l APalatin BH# jttlR%q%BHli4%%fl:e 3.16 tRNA~eu~kQdWflW#4f!il?il. 3.17 18s rRNAl%@ 118s RNA %a. 3.18 CaMV 35S135S promoter from cauliflower mosaic virus,?f€a T8@%%lJt$i%l8J3 5 S B a T . 3.19 NOS, terminator of nopaline synthase gene,M R B ~ - l i l t.~ ~