《SNT1203-2003-食用油脂中转基因植物成分定性PCR检测方法》.pdfVIP

Protocol of the qualitative polymerase chain reaction for detecting genetically modified plant components in edible oil T~UXf++8tl%~~*~SRtsfi5IH~B%*6@HA@RS 1#9HSIHX#t#M,GEJ?% HB$(Q%~iBHb@)%Bi5~%;BHT*ERt%Thitgh@@%*ER%BB~H83HR E 3 W @ H j h 3 4 r X # H S % M * o RE$ H#9H511X#t#t:a%M*3HT*6@o GB/T 6682 j ) ~ g ! S J # ~ # @ ~ B f j - ( e q vI S 0 3696: 1987) S N / T 1193 S N / T 1194 ~ @ W ~ F % ~ B B % @ M M # I # r ~ 1 # 2 i % S N / T 1195 kS+ffSBB%SBPCR 8!!2it S N / T 1196 33R+ffSB%Z# PCR B@l3% S N / T 1197 ?%H+ffBB%SBPCR BM2i!i- S N / T 1199 #j%+%Hfi%Z#@B!lPCR 3% S N / T 1202 .$t+%SB@%Z# PCR W!!I3% S N / T 1204 $hW#$QIFaa +%SBB%AB$?Efi PCR B@4$2i% T3U#i~%SHT*h5Ro 3. 1 PCR: polymerase chain reaction, m#PCR. 3.2 DNA: deoxyribonucleic a ~ i d , N R @ @ j @ ~ 3. 3 dNTP: deoxyribonucleoside t r i p h o s p h a t e , a g @ % Z @ @ . 3. 4 dATP: deoxyadeNOSine triphosphate ,%!@.8$%=€#@ 3. 5 dCTP:deoxycytidine triphosphate,$!glfi%3€#@., 3 . 6 dGTP: deoxyguaNOSine triphosphate ,li#RsffZ@@. 3.7 dUTP:deoxyuridine triphosphatel~~Jj25jff:€#@ 3 . 8 bp: base pair, Sjt;f. 3 . 9 Taq88i:NNAB88. 3. 10 Tris: tris( hydroxymethyI)aminomethane,3(~PS)$ilaSPj$., 3. 11 TE:Tris-CI.EDTA %#$. 3. 12 Lectin:B@@%%l. 3. 13 ZEIN:$QB$%EQ 3. 14 PE3-PEP case: phosphoenilpyruvateCarboxylase,@@#€!$fiBA@ftkI I. 3. 15 tRNALeu:B@W%#SiH 3. 16 CaMV 35s: 35s promoter from cauliflower mdsaic virus ,*$ TE##%EWSic55 35s G33. 3. 17 NOS: terminator of nopaline synthase gene,fdBB#-$?BiB%It3. 3. 18 FMV35S135Spromoter from a modified figwort mosaic virus (caulimovirus group), xEW I %t35SB325;b3. 3. 19 NPTII: neomycin-3-phosphotransferasegene ,%S!#-3-@BS@BS H . 3.20 BAR: phosphinothricin acet~ltransferasegene from Bacillus amyloli