BMSCs和EPCs移植对大鼠脑缺血后Bcl-2、Bax蛋白表达的影响.pdfVIP

4. 脑组织切片HE染色显示：B组组织明显水肿，神经细胞体积增大，空泡变性， 细胞间隙增大；E组神经细胞数量多,胞浆丰富,细胞受损程度在各组中最轻,低倍镜下 可见散在的小软化灶，其余组织结构相对较好。 结论 1．骨髓间充质干细胞和血管内皮祖细胞移植治疗缺血性脑损伤可以促进神经功 能的恢复，联合移植效果优于单细胞移植。 2．骨髓间充质干细胞和血管内皮祖细胞移植治疗缺血性脑损伤的作用机制之一 可能是通过上调 Bcl-2蛋白表达 ,下调 Bax蛋白表达,减少神经细胞凋亡。 关键词 骨髓间充质干细胞；血管内皮祖细胞；大脑中动脉梗塞；移植；Bcl-2；Bax 硕士研究生：陈芬芳 导 师：汤永红教授 3 Effects of BMSCs and EPCs transplantation and expressions of Bcl-2,Bax in rat cerebral ischemia-reperfusion tissue Abstract Objective： This study used bone marrow stromal cells bone marrow stromal cells, (BMSCs), endothelial progenitor cells (endothelial progenitor cells, EPCs) transplants Intervened the rat brain ischemia-reperfusion mode.To investigate the expressions of Bcl-2,Bax and the effect on bone marrow stromal cells(BMSCs) and endothelial progenitor cells(EPCs) transplantation for treating cerebral ischemia in rats. Methods: Rat middle cerebral artery occlusion(MCAO) model was established.A total of 100 SD rats were divided into sham operation group(A group),operation no treating group(B group),BMSCs(tail vein)group ,EPCSgroup and BMSCs +EPCScomplex group at random. A group of anesthesia, only isolated to the carotid artery, suturing the skin that is not any treated.B group is MCAO rat model.Ischemia reperfusion 2h, 7day after preparation of the model made, group training, respectively, BMSCs, EPCs dissolved in phosphate buffer solution, cell number was adjusted to 2 × 106 / L. C group is transplanted 2 × 106 / L BMSCs into the MCAO tail vein. D group is transplanted 2 × 106 / L EPCs into the MCAO tail vein.E group is transplanted 2 × 106 / L EPCs and2 × 106 / L BMSCs into the MCAO tail vein. Transplantation in each group equal