普罗布考防治大鼠顺铂肾毒性的实验研究肾病内科专业论文.docxVIP

硕 士 硕 士 学 位 论 文 英 文 摘 要 A B S T R A C T O bj ectiv es: To ev alu ate the relation sh iP am on g ox id ativ e stress， nu e le ar tra n sc riP tio n fa c to r S P I an d e e ll ap o P to s is in ki dn ey d am ag e rats in du e e d b y e isP latin : P ro b u e o l w a s a d m in iste re d to e o n firm its P ro te ctiv e ro le ag a in st n eP hr oto x ic ity in du e e d b y e isP latin . M e th o d s : T w en ty fo u r m a le a d u lt S P ra g u e一D aw ley rat s we re ran d om ly d iv id ed into fou r grouP s(6 rats in eaeh gr o uP )，n am ely n o rm o l co ntr o l(C N )，eisP latin (C P )，cisP latin + Pr o bu eo l(C P + P rob u eo l)，P rob u co l c o n tro l ( P ro b uc o l) . E x e P e et C p + P ro b u c o l an d P ro b u e o l gr o uP s re e e iv e d l % P ro b u c o l d ie t ，o th e r gr o uP s re e e iv e d n o rm a l d ie t u n til th e e n d o f tri a l. In 14th d ay o f exP erim ent，C N an d P robu eo l grou P s we re injeeted intr aP eriton eally (i·P .) w ith salin e lm l/2 0 09 ，C P an d C P + P ro b u eo l gr ouP s reeeiv ed sin g le d o se o f cisP latin 6m g/k g l.P 二C o lleetion the 24 h urin e o f rats before injeetion cisP latin 24 h an d 2 4h after injeetion ， m easu rem ent o f u rin ary N 一A cetyl一p 一D 一 g lu eo sam in id ase(N A G )，after injeetion cisP latin 4 8 h ，u se 10 % eh lo ral h y drate an esthesia rat s，th en P iek ey e to co lleetio n b lo o d m easu rem en t o f seru m ereatin in e(S er) an d b lo o d u rea n itro g en (B U N )，T h e eXten t o f liP id P ero x id atio n in the kidn ey w as ev alu ated by m easur in g m alon d iald e勿 d e(M D A ) an d g lu tath ion e p erox id e(G S H 一p x ) in the k idn ey tissu e h om o g en ate·Th e ren al inju ry W as a lso a sse sse d b y h e m ato xy lin a n d e o sin an d T d T-