人工控温促熟条件下不同海域菲律宾蛤仔性腺发育的研究-水产学报.DOCVIP

人工控温促熟条件下不同海域菲律宾蛤仔的性腺发育 李霞1 , 梁峻1 , 闫喜武1 , 张国范2 1大连水产学院，辽宁大连116023 2中国科学院海洋研究所，山东青岛266071 关键词 菲律宾蛤仔；性腺发育；不同海域；有效积温 中图分类号：Q954.6 文献标示码：A The relationship between water temperature and periodic variation of histology with gonad development of Manila clam Ruditapes philippinarum in different region LI Xia1, LIANG Jun1, YAN Xi-wu1, ZHANG Guo-fan2 （1.Dalian fisheries University，Dalian 116023，China； 2.Institute of Oceanology，Chinese Academy of Sciences，Qingdao 266071，China） Abstract： Manila clam, Ruditapes philippinarum, is one of the most important clam species cultured in China. By observing gonadal development of Manila clam from different areas under controlled water temperatures, we could forecast and control the spawning time. Manila clam was sampled in Dalian and Fujian in March，2004．Wamed them in indoor ponds to ripe with the same condition．Shell size and weight were measured．We reseached the histology of its gonad develepment and the relationship between periodic variation of histology with gonad development and effective accumulated temperature．The gonads were fixed with Bouin’s solution，and tissue sections were stained with H·E solution．Taking micrography pictures to show the gonad development of the Manila clam in Dalian was divided into five sections：spent stage，early active stage，later active stage，mature stage，spawned stage．The gonad development of the Manila clam in Fujian was also divided into five sections：early active stage，later active stage，mature stage，spawned stage，degegerating stage．The relationship of gonad develepment with effective accumulated temperature was discussed in this paper．It was indicated that the effective accumulated temperature of gonad development of the Manila clam in Fujian and Dalian were 190.1℃?d and 331.8℃?d，respectively． Key words: Ruditapes philippinarum；gonadal development；different region；effective accumulated temperature 资助项目：中国科学院知识创新工程课题（ZKCX2-211）国家863计划（2005AA603610） 作者简介：李霞（1961、11），女，江苏南京人，教授，从事水产动