hacat细胞共培养法诱导兔骨髓间充质干细胞表达角蛋白的研究烧伤整形外科专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 结论 通过与HaCaT细胞共培养兔骨髓间充质干细胞可表达角蛋白，并 可能被诱导分化为表皮细胞或表皮干细胞。 关键词骨髓间充质干细胞；角蛋白；HaCaT细胞 Ⅱ 硕士学位论文 硕士学位论文 英文摘要 ABSTRACT Objective To Investigate the possibility of inducing MSCs(marrow mesenchymal stem Cells)express cytokeratin by co-culture with HaCaT cell in vitro． Methods 1．The MSCs were harvested from bone marrow of rabbit,purified by Ficoll—Paque．The identification of MSCs was by detecting CD34，CD44, CD45 with flow cytometry． 2．3．passage MSCs and HaCaT黼co-cultured in flask(105：102 ／瑚)．After 3day and 7day,we divided’MSCs and HaCaT by different speed of trypsin digestion,then detected pan-cytokeratin and CKl9 by immunocytochemistry respectively． Results (1)Flow cytometry showed that the MSCs were positive in CD44，but did not expresse CD34 and CD45． (2)After 3day and 7day of co-culture，immunocytochemistry revealed mat both Pan-cytokeratin and CKl 9 were positive respectively．The positive rate(Pan-CX)of the MSCs that co-culture with HaCatT for 3aay(13．5+3．7％)were lower than the MSCs that CO—culture 嘶m HaCatT for 7day(29．14-8．3％，pO．01)significantly，nevertheless， the positive rate(CK 1 9)of MSCs that CO—culture with HaCatT for 3day TH 硕士学位论文 硕士学位论文 英文摘要 (3．1i-O．5％)was low,but compared with the MSCs that CO．culture with HaCatT for 7day(3．3i-0．8％，pO．05)，the variance was non-significant． Conclusion MSCs can express pan-ck and CKl 9 via co-culture with HaCaT，MSCs have the potential to differentiate into both epidermal cells or epidermal stem cells． Key Words：Marrow Mesenchymal Stem Cells，Cytokeratin，I-IaCaT cell IV 硕士学位论文 硕士学位论文 缩略语表 缩略语表 MSCs 骨髓间充质干细胞 FBS 胎牛血清 DⅣ匝M DulbeCCo’s Modificd Eagle’s Medium DMSO Dimcthyl sulfoxide 二甲基亚砜 ED弘 二乙胺四乙酸 PBS 磷酸盐缓冲液 髓 苏木精和伊红 CK 角蛋白 DAB 二氨基联苯胺 CD 人白细胞分化抗原 DMEM／F12 Dulbec圮,o’s Modified Eagles M锐litmdFl2 DMEM／F12培养基 Ⅵ 原创性声明本人声明，所呈交的学位论文是本人在导师指导下进行的研究工 原创性声明 本人声明，所呈交的学位论文是本人在导师指导下进行的研究工 作及取得的研究成果。尽我所知，除了论文中特别加以标注和致谢的 地方外，论文中不包含其它人已经发表或撰写过的研究成果，也不包 含为获得中南大学或其它单位的学位或证书而使用过的材料。与我工 作的同志对本研究所作的贡献均已在论文中作了明确的说明。 作者签名：继 学位论文版权使用授权书 本入了解