蔬菜中微量铜的测定方法.PDFVIP

258 2009, Vol. 30, No. 18 食品科学 ※分析检测 1 2, 李志熙 ，王瑞斌 * (1.榆林学院生命科学学院，陕西榆林 719000 ；2.榆林学院化学与化工学院，陕西榆林 719000) 建立测定蔬菜中微量铜的硫脲-EDTA 滴定新方法。样品经干法消解，在用溴化钾掩蔽汞干扰后的pH5 ～ 6 的消化液中，以砒啶偶氮萘酚为指示剂，用EDTA 标准溶液滴定能与EDTA 络合的所有金属离子的总量；然后 在另一份用硫脲掩蔽Cu2+ 后的试液中，再用EDTA 滴定除Cu2+ 外的其他金属离子。两者之差即相当于铜的量。测 定结果的相对标准偏差均小于2.50% ，其加标回收率在96.8%～100.4% 之间。结果表明，改进后的方法具有简单、 快捷、干扰小等特点。 蔬菜；铜含量；测定方法 Titration Determination of Trace Copper in Vegetables 1 2, LI Zhi-xi ，WANG Rui-bin * (1. College of Life Science, Yulin University, Yulin 719000, China； 2. College of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, China) Abstract：This study aimed to establish a new method using thiourea and EDTA titration for the determination of trace copper in vegetables. Samples were dry-digested, and KBr was then added to one portion of the digestion solution (pH 5－6) to mask Hg2+, and the total metal ions were tested by titration with standard EDTA solution using pyridylazo naphthol as an indicator. Subsequently, other metal ions except Cu2+ masked by thiourea in another portion were titrated with standard EDTA solution. Finally, the amount of copper was calculated by the volume difference of EDTA solution expended in titration of the two portions. The relative standard deviation was less than 2.50% and the spiked recovery was 96.8% －100.4%. This method has many advantages including simple and fast operation and little interference. Key words ：vegetable ；copper content ；determination method 中图分类号：O655.25 文献标识码：A 文章编号：1002-6630(2009)18-0