综述支架及无支架条件下构建组织工程软骨的研究进展.pdf

726 2007 10 20 10 China J Orthop Trauma, Oct. 2007, V o.l 20, No. 10 王金良, 赵建宁 ( , 210002) ! 支架材料的研究是组织工程的研究热点之一, 软骨细胞复合培养给组织工程软骨的构建带来希望, 一的材料诸如胶原透明质酸壳聚糖纤维蛋白凝胶等已经证明可以与软骨细胞复合培养,两种或者多种材料复合可 以提高材料的性能, 更好地用于组织工程软骨的构建, 并满足软骨缺损修复的需要; 同样,在无支架情况下应用软骨细 胞聚集培养沉淀培养的方法,可以构建组织工程软骨,并给软骨缺损的修复带来新希望, 但目前的研究较少两者是 组织工程软骨构建的两 个主要方向 ! 组织工程; 软骨; 细胞培养; 支架; 综述文献 Progress in studying the construction of tissue engineering cartilage w ith or w ithout various scaffolds WAN G J inli ang, ZHAO J ianning. Clinical College of N anj ing, Southern M edical Universit , D ep artm ent of Orthop aedics, N anj ing General H osp ital of N anj ing Command, N anj ing 210002, J iangsu, China ABSTRACT The study of scaffold is one of the focuses in tissue engineering, scaffold is cultivatedwith cartilage cells to con struct tissue engineering cartilage. A single scaffold, such as collagen, hyaluronic acid, chitosan, fibrin gel, et al, has been proved to succeed in cultivationw ith cartilage cell. Theperformance of scaffold could be mi proved by combining differentmate rials andmeet the need of repairing cartilage defects. Themethods of cartilage cellaggregation culture and pept culturew ithout scaffold has been proved to succeed in constructing tissue engineering cartilage and it brings new hope for people. owever few studies are available. The twomethods ofconstructing tissue engineering cartilage are two trends of cartilage tissue engineering. K ey words T issue engineering; Cartilage; Cellculture; Scaffold; Review literature Zhongguo Gushang/China JOrthop Trauma, 2007, 20( 10): 726728 www. zggszz. com 20 60, ∃ , Stark [ 5] , % , 2 ,