长柱重楼中主要甾体皂苷类成分的定性定量分析.docxVIP

长柱重楼中主要甾体皂苷类成分的定性定量分析 　　[摘要]为了从化学成分上研究长柱重楼是否具有重楼药材代用品的开发价值，该研究利用UPLCQTOF MS比较了长柱重楼和滇重楼的化学成分，并进一步利用HPLCUV同时测定了77株不同来源的长柱重楼中8 种重楼皂苷的含量。结果显示长柱重楼和滇重楼的化学成分相似，长柱重楼化学成分更为复杂，鉴定的22个主要共有峰均为甾体类化合物。不同株长柱重楼中重楼皂苷的含量具有一定的差异，其中药典规定4种甾体皂苷总量最高的为330%，最低的为0068%，8种甾体皂苷总量最高的为618%，最低的为071%。长柱重楼中78%的样品符合《中国药典》要求，整体质量较为稳定，可以作为重楼替代品种进行研究开发。　　[关键词]重楼； 长柱重楼； 甾体皂苷； 定性定量分析　　Qualitative and quantitative analyses of primary saponins in Paris forrestii　　HUANG Yuanyuan1，2， KANG Liping1， PENG Huasheng2， LIU Dahui3， HAO Qingxiu1，　　ZHAO Jialiu4， CHEN Min1*， HUANG Luqi1*　　　　[Abstract]In order to study whether Paris forrestii could be developed as a substitute of Paridis Rhizome， chemical compositions of P. forrestii and P. polyphylla var yunnanensis were investigated by UPLCQTOF MS In addition， the contents of eight primary steroidal saponins in 77 batches of P. forrestii samples from different habitats were simultaneously determined by HPLCUV The results showed that P. forrestii and P. polyphylla var yunnanensis have similar chemical compositions， and all 22 major common peaks were identified as steroid derivatives Meanwhile， there were some differences in the contents of saponins in P. forrestii samples from different habitats The contents of 4 steroidal saponins in Chinese Pharmacopoeia ranged from 0068% to 330%， and the highest content of the 8 kinds of steroidal saponins was 618%， while the lowest was just 071% Moreover， 78% of P. forrestii samples were in conformity with the requirements of Chinese Pharmacopoeia， indicating that P. forrestii samples had relatively stable quality and could be further studied as a substitute for Paridis Rhizome　　[Key words]Paris； Paris forrestii； steroidal saponins； qualitative and quantitative analyses　　XX年版《中国药典》[1]记载重楼来源于滇重楼Paris polyphylla Smith var yunnanensis 和七叶一枝花P. polyphylla Smith var chinensis Hara的根茎。重楼中主要含有甾体皂苷类成分，目前已报道的有160余种，这类成分具有止血、抗肿瘤、抗菌、消炎、镇痛等药理活性[27]。重楼是云南白药、宫血宁、金复康口服液、楼连胶囊和季胜德蛇药片等中成药的主要组成药味[8]，具有显著的临床疗效和经??价值。　　李恒等对中国重楼资源现状进行了评估，发现重楼消