肾阳虚对试验大鼠胃窦小肠黏膜组织显微结构的.pdfVIP

国际中医中药杂志2013年6月第35卷第6期 Int J Trad Chin Med, June 2013, Vol. 35, No.6 ·507 · ·实验研究 · 肾阳虚对实验大鼠胃窦、小肠黏膜组织 显微结构的影响 吕银娟 张智华 吴建红 许湘 周安方 张玉辉 【摘要】 目的 探讨肾阳虚对实验大鼠胃窦、小肠黏膜组织显微结构的影响。方法 切除大鼠双 侧肾上腺的 3/4 复制肾阳虚动物模型，术后 4 周分为肾阳虚模型组、参附汤组，假手术组除不切除肾上腺 外余同手术组，作为对照组。参附汤组大鼠灌胃给予参附汤水煎液，肾阳虚模型组和对照组给予等容量 的生理盐水，灌胃 4 周。常规 HE 切片观察各组大鼠胃窦、小肠黏膜组织结构。结果 ①肉眼观察，假 手术组大鼠的胃肠黏膜正常，呈粉红色，有光泽，弹力较好；与假手术组比较，模型组大鼠的胃肠黏膜 变薄，色泽暗淡，弹力较差；与模型组比较，参附汤组大鼠的胃肠黏膜稍厚，略有光泽，弹力尚可。② 显微镜下可见，假手术组大鼠的胃窦、小肠黏膜组织结构正常；与假手术组比较，模型组大鼠胃窦黏膜 组织细胞间隙变大，黏膜腺体有不同程度的排列紊乱、疏松，出现大量空泡样变；小肠黏膜绒毛边缘不 整齐，部分上皮细胞脱落、缺失；与模型组比较，参附汤组大鼠胃窦黏膜组织细胞间隙变小，腺细胞排 列较规则，空泡样变减少；小肠黏膜组织绒毛排列规则，上皮基本完整。结论 肾阳虚证动物模型胃窦、 小肠黏膜组织结构出现病理改变。 【关键词】 肾阳虚；显微结构；实验研究 The influence of kidney-yang deficiency on rat’s gastric antrum and small intestinal mucosa tissue microstructure change * LÜ Yin-juan , ZHANG Zhi-hua, WU Jian-hong, XU Xiang, ZHOU An-fang, ZHANG Yu-hui. *College of basic medical, Hubei university of Chinese medicine, Wuhan 430065, China Corresponding author：ZHOU An-fang, Email ：zhouanfang@21 【Abstract 】 Objective To research the influence kidney-yang deficiency on rat ’s gastric antrum and small intestinal mucosa tissue microstructure ’s change. Methods The rats were cut off bilateral adrenal three-quarters to establish the model rats with deficiency of kidney-yang, which were randomly divided into a model group and a Shenfu decoction group at 4 weeks after operation. The sham operation group was the same as model group except without cutting rats bilateral adrenal and setting as a control group. The Shenfu decoction group was given suspension liquid of Shenfu decoction by lavage, and both the control group and model group were given physiological saline