富含维生素C食品中总黄酮含量的测定-食品安全质量检测学报.PDF

6 5 Vol. 6 No. 5 2015 5 Journal of Food Safety and Quality May. , 2015 C * * 虞哲高 , 林冰东 , 邢月寒, 王晓燕 (, , 201203) 摘 要: 目的 C , C 方法 Waters Sep-Pak C18 , D101 (SPE) C, , , 510 nm结果 0~48 µg/mL , 0.9998, (RSD) 5%, 95.31%~ 98.38%C (Vc:=1:1, 2:1, 10:1), C , ,, 结论 , C 关键词: C; ; ; D101 ; Determination of total flavonoids in rich ascorbic acid food * * YU Zhe-Gao , LIN Bing-Dong , XING Yue-Han, WANG Xiao-Yan (DSM Nutritional Products China Research Center, DSM China Co ., Ltd., Shanghai 201203, China) ABSTRACT: Objective To establish a quantitative analysis of total flavonoids with high content of ascorbic acid(vitamin C) in food and solve the interference of ascorbic acid on the measurement of total flavonoids by complexation reaction. Methods A solid phase extraction procedure was developed to degrease and remove pigments by Sep-Pak C 18, vitamin C was removed with D101 macro-porous resin, total flavonoids were determined by aluminum nitrate colorimetric using detection wavelength at 510 nm. Results The developed method was successfully used to determine total flavonoids in vitamin C rich fruits/plant extracts. It showed a good linea