铁离子在大鼠试验性脑室出血后脑积水发生中的作用-第三军医大学学报.DOCVIP

PAGE PAGE 4 铁离子在大鼠实验性慢性脑积水发生中的作用 宫国旗，孟 辉，冯 华（第三军医大学西南医院神经外科, 全军神经系统疾病微创诊治专科中心，重庆 400038） 摘要 目的 研究铁离子在大鼠实验性慢性脑积水发生中的作用。方法 SD雌性大鼠75只，采用随机数字表法分为3组：铁离子组、半量铁离子组及人工脑脊液组。分别将浓度为6.67 g/L，3.33 g/L三氯化铁溶液及人工脑脊液各5 μl注入大鼠右侧侧脑室，于术后1、14 d及28 d处死大鼠，评价脑积水发生情况并测量侧脑室侧室指数（侧脑室面积/脑片面积×100），观察脑组织病理变化。结果 28 d铁离子组9只大鼠发生脑积水（n＝10），半量铁离子组1只大鼠发生脑积水（n＝5），两组相比差异显著(P 0. 01)，人工脑脊液组未出现脑积水。28 d铁离子组侧室指数(6.87 ±2.19)大于半量铁离子组(3.32 ± 0.95)及人工脑脊液组(1.09 ±0.45)（P 0. 01)。铁离子组室周脑组织细胞增生、出现大量泡沫状细胞、脉络丛水肿、含铁血黄素的脑室壁沉积，后期出现脉络丛萎缩、室管膜细胞数量减少，纤毛脱落，半量铁离子组相对较轻，人工脑脊液组无明显变化。 结论 侧脑室注入高浓度铁离子可诱发脑积水。 关键词 铁；脑积水；大鼠 The Role of Iron in Experimental Hydrocephalus in Rats GONG Guo-qi , MENG Hui, FENG Hua( Department of Neurosurgery, Southwest Hospital, Third Military Medicine University, Chongqing 400038, China) Abstract Oobject To investigate the roles of iron in experimental hydrocephalus in rats. Methods 75 Sprague Dawley female rats were divided into 3 groups randomly: the iron group, the semis-iron group and the articial CSF control group. 5 μl FeC13 dissolved in artificial CSF with the concentration of 6.67 g/L or 3.33 g/L were slowly infused into the right lateral cerebral ventricle of the rats in the iron group and in the semis-iron group. The rats in the articial CSF control group were infused 5 μl articial CSF . The rats were sacrificed 1, 14 and 28 days for measurement of the transverse diameter of the lateral ventricle for the evaluation of hydrocephalus and the index of ventricle, and pathology of brain was also observed. Results There were significant difference of hydrocephalus between in the iron group(9/10) and that in the semis-iron group(1/5) at 28 d(P 0. 01). The index of ventricle in iron group were higher than the semis-iron group and the articial CSF control group(P Key Words Iron; Hydrocephalus; Rats 脑积水是脑出血后的常见并发症，据统计高达50%的自发性脑出血合并脑室出血，其中35%会发展为脑出血后脑积水。目前虽然有脑脊液分流手术和三脑室底造瘘手术, 但总体治疗效果仍然不佳[1,2]。由于对后做到早期防治。但有关脑出血的研究证实：血液 基金项目：国家“十一五”科技支撑计划资助（2006BAI01A12） Supported by the “Eleventh Five-year Plan” for Sc