降温速率对溶液结晶的影响.PDFVIP

降温速率对溶液结晶的影响 李玉芳1，赵刚2 （1.中国科学技术大学电子科学与技术系，合肥市 230027） （Tel: 0551 Email: zhaog@ustc.edu.cn） 摘 要：在常见的低温保存及冷冻过程中，保护剂的种类和降温速率影响着最终的保存效果。本实验借助了现有的冷热台 与低温显微镜条件，研究了不同降温速率（5、20 、60 C/min ）条件下不同溶液结晶温度的变化。通过一系列实验发现：对 于同一溶液，随着降温速率的增大，溶液的结晶温度会降低；对于不同溶液，降温过程中冰晶的形态也是各不相同，生长尖 端也是有些尖锐有些平缓。该实验结果表明溶液种类和降温速率对溶液的结晶温度以及冰晶形态存在一定的影响，在以后的 实验中，应该针对不同的要求选用不同的保存溶液和降温速率以实现最好的效果。 关键词：低温保存；降温速率；冰晶形态；结晶温度 Effect of Cooling Rate on Crystallization of Solution Li Yufang1 Zhao Gang2 (1. Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027) Abstract In the common cold storage and freezing process, the type of protective agent and the cooling rate affect the final preservation effect. In this experiment, the crystallization temperatures of different solutions under n) were studied by using the existing hot and cold table and cryogenic microscope conditions. Through a series of experiments, it was found that for the same solution, as the cooling rate increases, the crystallization temperature of the solution will decrease; for different solutions, the morphology of the ice crystals during the cooling process is also different, and the growth tip is also somewhat sharp and somewhat flat. The experimental results show that the type of solution and the cooling rate have a certain influence on the crystallization temperature and the ice crystal form of the solution. In the subsequent experiments, different storage solutions and cooling rates should be chosen to achieve the best results for different requirements. Keywords Cryopreservation Cooling rate Ice crystal form Crystallization temperature 0 前言 现代低温生物医学中，细胞、组