职业接触苯乙烯工人静脉血中蛋白质环氧苯乙烯加合物-环境科学学报.PDFVIP

20 4 Vol.20, No. 3 2000 7 ACTA SCIENT IAE CIRCUMSTANT IAE July, 2000 : 2000 : X502, X503. 1 : A 1 1 1 2 刘淑芬, 方清明, 金祖亮 , Rappaport, S. M. ( 1. , 100085; 2. UNC AT CHAPE HI NC USA) : ( SO) , Raney Ni :,, , GC/ MS . SD , SO ; SO . , , SO + ,; . SO . : ; ; Detection of protienstyrene oxide adducts in human blood exposed to styrene IU Shufen, FANG Qingming, JIN Zuliang ( Research Center for Ecoenvironmental Science, Chinese Academy of Sciences, Beijing 100085) Rappaport M S ( UNC at Chapel Hill. NC. USA) Abstract:An assay is described for measures of adducts of styrenceoxide ( SO) , with molecules of protein which have been modified at residues containing sulhydryl group. Sample is hydrolyzed, then and reacted with Raney Ni to liberate the two positionisomer of SO cysteine adducts, i. e. , 1phenylethanoland, 2phenylethanol. The liberated products are derivatized with pentafluorobenzoyl chloride and measured by GC/ MS. T he assaywas evaluatedwith SO adducts of Hb which hadbeen produced in blood of SD rat and in hurman blood exposed to styrene. evel of proteinSO adducts in rat blood increased with dose and theposition rather that the position both. The two adducts were found in workers blood exposed to styreneand there is a good relativity between adduct level and exposed doseof styrene. . It is suggested that SOprotein adduct can be a biomarker of styrene exposure in workers. Keywords:protein adduct; styreneoxide , , , , . [ 1] [ 2] , , , . [3] DNA DNA , , DNA , DNA . , , , : : : ( : ; : , , 462