AIVNP基因在噬菌体表面的展示及间接ELISA方法的建立.PDFVIP

2008, 39( 11) : 1537-1543 Acta Veterinaria et Zootechnica Sinica AIV NP ELISA 1,2, 3 1* 2* 1 1 1 , , , , , ( 1. , 510642; 2. , 100101; 3. , 450002) : DNA , ( AIV) T 4-z1, T4-z1-NPSDSWester blot , AIV T4 T4- z1-NP (T4-NP-ELISA) ( AGP) 100% , 871 62% , T 4-NP-ELISA 911 43% T4-NP-ELISA H 5 H7 H 9 AIV , IBDIBMDNDEDS , 1B 640 , 192 , T4- NP- ELISA IDDEXX ELISA 961 8% T4-NP-ELISA AIV , AI : ; ; T4 ; ; T4-NP-ELISA : S8521 6591 5; Q78 : A :0366-6964( 2008) 11- 1537-07 Display of NP Gene of AIV on the S rface of Bacteriophage T4 and Development of Indirect ELISA on the Basis of the Expressed NP Protein 1, 2, 3 1* 2* WANG Xi -w ei , BI Yi g-zuo , HE Ho g-xua , 1 1 1 WANG Xia -w e , ZHAN A-i ju , MA Ji g-yu (1. College of A nimal Science, South China A gricultural University , Guangz hou 510642, China; 2. Institute of Zoology , Chinese A cademy of Sciences, eij ing 100101, China; 3. College of A nimal H usbandry and Veterinary, H enanA gricultural University , Zhengz hou 450002, China) Abstract: A 1 320 bp fragme t of NP ge e of avia i flue za virus ( A IV ) w as clo ed a d i te- grated i to the ge ome of T4-z1 phage w ith SOC-deleted a d lysozyme-defected by homologous recombi atio . T he recombi a t phage w as amed as T4-z1-NP. T he results of SDS-PA GE a d Wester blot show ed that ucleoprotei of AIV w as successfully displayed o the surface of the T4-z1-NP. Based o the purified T4-z1