脱细胞猪小肠粘膜下层基质资料中生长因子定量检测.pdfVIP

Material Sciences 材料科学, 2018, 8(5), 595-602 Published Online May 2018 in Hans. /journal/ms /10.12677/ms.2018.85070 Quantitative Detection of Growth Factors in Porcine Small Intestinal Submucosa Matrix Materials 1 1 2 1 1 1 Yang Zhang , Leilei Xia , Jianping Gao , Fumin Men , Xiaolong Zheng , Jinhui Zhang , Yi Chen1*, Bo Zhao1* 1 Beijing Biosis Healing Biological Technology Co., Ltd., Beijing 2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing th th th Received: May 4 , 2018; accepted: May 20 , 2018; published: May 28 , 2018 Abstract In this study, three growth factors including basic fibroblast growth factor (FGF2), transforming growth factor β1 (TGFβ1) and vascular endothelial growth factor (VEGF) in two SIS products (VIDASISTM and Biodesign®) were quantified by enzyme linked immune sorbent assay (ELISA) kit through the preliminary treatment of liquid nitrogen freezing and grinding technology which was aimed to release the growth factors from the tissue. Results showed that contents of TGFβ1, VEGF and FGF2 in VIDASISTM were 8375 ± 2125 pg/g, 5486 ± 1043 pg/g and 3990 ± 1372 pg/g respec- tively; while the corresponding contents in Biodesign® were 11517 ± 1331 pg/g, 5432 ± 272 pg/g and 5417 ± 947 pg/g. Keywords Porcine Small Intestinal Submucosa, Acellular Matrix, Transforming Growth Factor, Basic Fibroblast Growth Factor, Vascular Endothelial Growth Factor 脱细胞猪小肠粘膜下层基质材料中生长因子的 定量检测 1 1 2 1 1 1 1* 1* 张 扬 ，夏磊磊，高建萍，门福民，郑晓龙，张晋辉，陈 毅 ，赵 博 1北京博辉瑞进生物