乙酸分级预处理甘蔗渣对纤维素酶解性能的影响.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2011, March 25; 27(3): 384−392 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2011 CJB, All rights reserved. 燃料乙醇技术 乙酸分级预处理甘蔗渣对纤维素酶解性能的影响 赵雪冰，刘德华 清华大学化学工程系应用化学研究所，北京 100084 摘 要: 为提高甘蔗渣的纤维素酶解性能，采用乙酸脱木素结合碱脱乙酰基的预处理工艺 (Acetoline 工艺) 对甘蔗渣进 行预处理，考察了乙酸脱木素过程中若干因素对预处理结果的影响，并对预处理后甘蔗渣的纤维素酶解性能进行了研 究。结果表明，经过Acetoline 预处理后甘蔗渣在7.5 ％固体含量、15 FPU+10 CBU/g 固体的纤维素酶和β- 葡萄糖苷酶 用量下酶解48 h ，酶解聚糖转化率接近80 ％。与稀酸预处理相比，Acetoline 预处理可以得到更高的酶解聚糖转化率。 实验结果表明Acetoline 工艺是一种可有效提高甘蔗渣纤维素酶解性能的预处理方法。 关键词: 木质纤维素，预处理，酶解性能，脱木素，脱乙酰基 Fractionating pretreatment of sugarcane bagasse for increasing the enzymatic digestibility of cellulose Xuebing Zhao, and Dehua Liu Institute of Applied Chemistry , Department of Chemical Engineering, Tsinghua University, Beijing 100084, China Abstract: During the bioconversion of lignocellulose to ethanol, the biomass always undergoes pretreatment in order to increase the enzymatic digestibility of cellulose. In present work, we conducted the pretreatment of sugarcane bagasse with aqueous acetic acid for delignification and alkali for deacetylation respectively (Acetoline process) to increase cellulose accessibility for enzymatic hydrolysis. The effects of several factors on the pretreatment effectiveness were investigated. The enzymatic digestibility of pretreated bagasse was further studied. The enzymatic glycan conversion of pretreated solid reached about 80% when it was digested under 7.5% solid consistency with cellulase of 15 FPU/g solid and β-glucosidase of 10 CBU/g solid for 48 h. Compared with dilute acid pretreatment, Acetoline pretreatment could obtain higher enzymatic glycan conversion. The e