水稻极矮突变体s47对赤霉素的响应及基因定位研究-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2013, 39(10): 1766−1774 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.01766 水稻极矮突变体s2-47 对赤霉素的响应及基因定位研究 1,2 1 3 2 3 1,* 2,* 李晨晨 侯 雷 尹 亮 赵金凤 袁守江 张文会 李学勇 1 , 252059; 2 / , 100081; 3 , 250100 : EMS 1 s2-47, α-, s2-47 GA , GA3 s2-47 s2-47 Dular F2 , s2-47 OsCPS1 , , s2-47 OsCPS1 OsCPS1 , OsCPS1 GA3 , s2-47 : ; ; ; ; OsCPS1 Gibberellin Responsiveness and Gene Mapping of Rice Extreme Dwarf Mutant s2-47 1,2 1 3 2 3 1,* LI Chen-Chen , HOU Lei , YIN Liang , ZHAO Jin-Feng , YUAN Shou-Jiang , ZHANG Wen-Hui , and LI Xue-Yong2,* 1 School of Life Science, Liaocheng University, Liaocheng 252059, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shandong Rice Research Institute, Jinan 250100, China Abstract: We have isolated a dwarf mutant s2-47 from Nipponbare mutagenized by EMS, which is an extreme dwarf with dark green leaves and without reproductive development. GA3 treatment of seedlings and α-amylase activity analysis in endosperm showed that the mutated gene is involved in GA biosynthesis. Fine mapping showed that the mutant phenotype was tightly linked with the OsCPS1 locus, which encodes the ent-copalyl diphosphate s