草莓未受精子房的离体培养-中国农学通报.PDF

中国农学通报 2011,27(13): 177-182 Chinese Agricultural Science Bulletin 草莓未受精子房的离体培养 1 2 1 王文和 ，吴禄平 ，赵玉萍 1 2 （北京农学院园林学院，北京102206；沈阳农业大学园艺学院，沈阳110161） 摘 要：花药培养和未受精子房或胚珠离体培养是人工诱导形成单倍体植株的2 条途径。草莓染色体倍 性复杂多样，为创新育种资源，本研究以草莓为试材，分析未受精子房的离体培养过程中基因型、外源激 素、蔗糖浓度、低温预处理、生长环境及发育状况、光照条件6 个关键因素对诱导雌核发育的影响。结果 表明：选择合适基因型的露地栽培的一级花蕾，外源激素添加2,4-D ，蔗糖浓度6% ，低温预处理48 h ，黑 暗变温处理是草莓未受精子房离体培养获得单倍体胚囊植株的理想条件。本研究为草莓倍性育种提供 参考。 关键词：草莓；未受精子房；雌核发育；离体培养 中图分类号：S668.4 文献标志码：A 论文编号：2011-0147 CultureinvitroofUnfertilizedOvaryinStrawberry(Fragariaspp.) WangWenhe,WuLupin,ZhaoYuping1 2 1 1 (College of Landscape Architecture, Beijing Agricultural,Beijing102206; 2College of Horticulture, Shenyang Agricultural University,Shenyang110161) Abstract: Anthercultureandculture in vitro ofunfertilizedovaryorovulearetwomethodstoartificially inducehaploidplantletsstrawberryarewithcomplicatedploidy.Forthepurposeofcreatingnovelbreeding materials,weemployedstrawberryasexperimentalmaterials,analyzedeffectsofsixkeyfactors,including genotype,extrinsichormone,sucroseconcentration,coldpretreatment,growthenvironmentanddevelopment status,andilluminationconditiononinductionofgynogenesisinvitroofunfertilizedovary.Resultsshowedthat theprimarybudoflandplantswithappropriategenetype, 2,4-Dasextrinsichormone,theconcentrationof sucroseatsixpercentageandcoldpretreatmentfor48hours,weretheoptimumconditionsforhaploidplantsby embryosacinductionof unfertilizedovaryinstrawberry.Theresearchaimedatprovidingreferenceforploidy breedinginstrawberry.