免疫比浊法定量检测葡聚糖的研究-甘蔗糖业.PDFVIP

甘蔗糖业 2013 年第 5 期，2013 年 10 月 Sugarcane and Canesugar, No. 5, Oct. 2013 免疫比浊法定量检测葡聚糖的研究 1 1 2 1 1 2 1,2* 柳 颖 ，蚁细苗 ，林荣珍 ，曾练强 ，黄曾慰 ，马 步 ，梁达奉 1 2 （广州甘蔗糖业研究所 广东省甘蔗改良与生物炼制重点实验室，广东广州 510316 ； 广西糖业研发中心，广西南宁 530002 ） 摘 要：葡聚糖影响甘蔗品质及制糖生产，主要表现在糖分损失、糖液粘度增加、过滤困难、结晶异 常、糖产品适用性受限等，从而降低提糖率，增加制炼成本，影响糖产品质量。本文对单克隆抗体比 浊法测定葡聚糖的方法学进行研究，结果表明，该方法能快速准确测定糖厂蔗汁等在制品及产品中葡 聚糖含量，线性范围 10 ～500 mg/L ，检测限为 10 mg/L，加标回收率 98 % ～102 %，RSD4 % ，灵敏度 1.3 mg/L。 关键词：葡聚糖；甘蔗品质；制糖；免疫比浊 中图分类号：TS241 文献标识码：A 文章编号：1005-9695(2013)05-0052-04 The Immunoturbidimetry Methods for Quantitative Detection of Dextran 1 1 2 1 1 2 LIU Ying , YI Xi-miao , LIN Rong-zhen , ZENG Lian-qiang , HUANG Zeng-wei , MA Bu , LIANG Da-feng1,2 1 ( Guangzhou Sugarcane Industry Research Institute/Guangdang Key Lab of Sugarcane Improvement Biorefinery, Guangzhou 510316; 2Guangxi Sugarcane Industry RD Center, Nanning 530002) Abstract: Dextran causes marked harmful impact on sugarcane quality and sugar production, which leads to severe sugar loss, increased viscosity of the flow, elongated crystals, expensive yield loss and declined quality of sugar. Based on the newly-generated monoclonal antibody, a reliable immunoturbidimetric method for quantitative detection of dextran was developed. This article studied the proformance of the method, which was proved to be a rapid, correct and convenient tool for detecting dextran in raw material, by-products and end-products o