芸苔属不结球叶菜种原总硫配醣体与产量及园艺性状关系之研究.DOC

蕓苔屬不結球葉菜種原總硫配醣體與產量及園藝性狀關係之研究 Analysis of Total Glucosinolate Content in Leafy Brassica Germplasm and Its Relationship with Yield and Selected Horticultural Traits 研究生：藍斯 Lance Ledesma 指導教授：賴博永 Lai,Po-Yung Peter Hanson 【摘要】 硫配糖體是普遍在蕓薹屬植物存在的天然二次代謝物。這些化合物是蕓薹屬植物中風味的主要來源，並且在植物防禦機制中占有重要角色。近期的研究顯示蕓薹屬植物之硫配糖體具有抗癌特性。為了瞭解蕓薹屬不結球葉菜硫配糖體總含量與變異性，試驗三種蕓薹屬作物之硫配糖體總含量。並評估作物的硫配糖體總含量與園藝特性之相關性。試驗樣品共有87個種原品種包括；白菜(Brassica rapa var. chinensis) (n=37)；油菜(Brassica rapa var. parachinensis) (n=22)和芥藍菜(Brassica oleracea var. alboglabra) (n=28)，分別在涼爽乾燥(11月到1月)和高溫潮濕(6月到9月)兩個季節栽種，並以微管柱-酵素分析法測定硫配糖體總含量，以及記錄園藝特性包括植株重量、植株高度、葉子長度、葉片長度和寬度和主要葉脈長度。研究的結果顯示硫配糖體總含量在品種間與種原品系間具有明顯著差異。季節性的變化也導致顯著性的差異。白菜在高溫潮濕的季節期間顯示有較高的硫配糖體總含量，平均值為19.95 μmole/g 乾重。而油菜與芥藍菜在涼爽乾燥的季節有高的硫配糖體總含量分別平均值為14.28 μmole/g乾重和23.45 μmole/g乾重。白菜在涼爽乾燥和高溫潮濕之二季，硫配糖體含量與產量之間顯示負的相關係數(分別為r = –0.43，p0.007 和r = –0.61，p0.001)，油菜則在涼爽乾燥的季節，硫配糖體含量與產量之間有高的相關係數(r = 0.62，p0.002)。而芥藍菜在測定之硫配糖體含量與產量間則無任何相關性。結果顯示不同的園藝特性與硫配糖體總含量的相關性隨著季節變化具有高變異性。 關鍵字︰蕓薹屬、微管柱-酵素分析法、硫配糖體、二次代謝物、產量、園藝特性 【Abstract】 Glucosinolates are secondary metabolites of plants occurring naturally throughout the family Brassicaceae. These compounds are mainly responsible for the flavor and odor of Brassica plants and play an important role in plant defense mechanism. Recent studies showed the involvement of glucosinolates in the anti-carcinogenic properties of brassicas. To understand the variability of total glucosinolates content in leafy brassicas, three Brassica crops representing two species for total glucosinolate content were evaluated. The possible relationship of total glucosinolate content with plant yield and selected horticultural traits was also evaluated. A total of 87 accessions representing pak-choi (Brassica rapa var. chinensis) (n=37), choy-sum (Brassica rapa var. parachinensis) (n=22) and kailaan (Brassica oleracea var. alboglabra) (n=28) were tested separately for two seasons, cool dry (November to January) and hot wet (June to September) and