8个禾本科草坪草品种遗传多样性的RAPD分析-亚热带植物科学.PDFVIP

2006,35(1):5-8. 8 RAPD 524048 RAPD 4 4 8 15 PCR RAPD 144 63.2%8 0.0857 0.2928 2 UPGMA 2 4 3 () RAPD Q943; Q949.71+4.2 A 1009-7791(2006)01-0005-04 Genetic Diversity Analysis on Eight Turfgrass of Poaceae by RAPD CHEN Yan, HU Mu-lin, YUAN Chang-chun, GUAN Guang-hui, LUO Mei, HUANG Yong-lian (College of Life Science and Chemistry, Zhanjiang Normal University, Zhanjiang 524048, Guangdong China) The genetic diversity of eight turfgrass varieties representing four species in four genera of family Poaceae was analyzed using random amplified polymorphic DNA (RAPD) method. Fifteen 10-meroligonucletide primers were used for DNA ampification, and 144 DNA bands were generated, of which 63.2% is polymorphic loci. The genetic distances are 0.0857~0.2928 among eight turfgrass varieties, the maximum value is between the variety Crossfire of Festuca arundinacea and Accent of Lolium perenne, the minimum value is between the two varieties of Unhulled Cynodon dactylon and hulled Cynodon dactylon. The two dendrograms based on genetic distance by UPGMA and Neighbor-joining method are similar topologically. The relationships among different varieties from the same species are very close. The relationships of inter-genera or interspecies are relatively far, although the different genera of the tribe Chlorideae get together into a clade. The four warm-season turfgrass form a clade. The variety Accent of Lolium perenne , a cool-season turfgrass, has a far relationship to other turfgrass and forms a clade. The study didn鈥檛 suppor