同一批次色谱柱不同批次色谱柱的重现性.pptVIP

Column-to-Column and Batch-to-Batch Reproducibility 同一批次柱子和不同批次柱子的重现性 Qustion 色谱柱 构造：由柱管和固定相组成，是色谱仪的最重要部件。 性能评价：柱性能指标包括：在一定实验条件（试 样，流动相，流速，温度）下的柱压，塔板高度H和板数n，对称因子，保留因子和分离因子的重复性或分离度R. good column-to-column reproducibility? Q.1: I am about to start the development of a new HPLC method. After validation, the method will be transferred to the QC-lab, where it will be used for many years. I am concerned about the long-term reproducibility of the column. What can I do to assure good reproducibility? 问：我要开始开发一个新的HPLC方法。验证后，该方法将转到QC实验室并将使用许多年。我担心该方法的长期的重现性，特别是柱子的长期重现性。 怎样才能确保好的重现性呢？ A.: First of all, let me compliment you for thinking about this aspect of your method before you start working on it. If it can be anticipated that a new method will be used for several years, this knowledge should be part of the column selection process. You may want to assure yourself that the column will be available for the anticipated life-time of the method. This means that you want to select a manufacturer that has a large operation and is likely to stay in business for the foreseeable future. In addition, you want to select a standard surface chemistry - like C18 or C8 - over novel and/or less used surface chemistries. In other words, your column selection should be conservative. The next criterion should then be the reproducibility of the column. If you or your colleagues have had good experience with the reproducibility of a particular column in the past that is a good starting point. You should also consider the information that is available from the manufacturer. Recently some column manufacturers have started to publish their specifications and the results of their batch-to-batch reproducibility studies. You can obtain this information from the manufacturers and review it critically. 怎样才能确保色谱柱好的重现性？ 包括： （1）选择一些大的生产厂家 （2）选择一种标准的表面化学物质 （3）考虑生产商提供的信息 the information from manufacturers ?  Q.2: What am I looking for in this information? 问:我该从这些信息中寻找什么呢？ A.: Let m