尖锐湿疣中PAR-1、β-catenin的表达及研究-皮肤与性病学专业论文.docxVIP

汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 II II 结果： CA 组 织 的 血 管 数 （ 20.81±9.09 ） 比 正 常 包 皮 组 织 （ 11.36±2.45 ） 明显增多 (t=5.192,P0.001)。CA组织中PAR-1 mRNA平均表达水平（1.541±0.1280）显著高于正常 包皮对照组（0.728±0.214，t=7.26，P0.001）；CA皮损中PAR-1蛋白表达较正常人表皮 中的表达显著增强（X2＝16.218，P0.001），正常人表皮中仅在基底层弱阳性表达，而在 CA皮损中PAR-1的表达为基底层至角质层下方。PAR-1蛋白表达跟血管数存在显著正相关 （r=0.823，P0.001）。β-catenin在正常组中的表达均定位于细胞膜，为正常表达，6 例（21.4%）CA皮损为细胞膜表达缺失，呈胞质和/或胞核异常表达，其异常表达率显著高 于正常组（X2=4.203，P0.05）。PAR-1蛋白的阳性表达与β-catenin的异常表达存在正相 关（P 0.05，r=0.101）。28例CA皮损中均可检测出HPV DNA，其中HPV6/11型23例，HPV16/18 型5例；而正常皮肤组织中HPV DNA 均为阴性。HPV16/18的阳性表达与β-cat的异常表达 存在高度正相关（P0.001,r=0.893）。 结论： CA 主要是由 HPV6/11 型感染所致；PAR-1 的过表达与β-catenin 的异常表达参与 CA 的发病。PAR-1 过表达和β-catenin 的异常表达均可能与 CA 中的细胞增殖有关。PAR-1 过 表达还与 CA 的血管生成有关。 关键词：尖锐湿疣、人乳头瘤病毒、β连环蛋白、蛋白酶激活受体-1 PAGE PAGE IV ABSTRACT Background and Objective： Condyloma acuminate, also called genital warts, is one of the most common sexually transmitted diseases. The incidence of the disease increases these years. Human papilloma virus (HPV) is etiologically associated with the development of genital warts. Over 100 subtypes of HPV are identified recently. Genital warts typically are caused by HPV subtypes 6, 11, 16 and 18. The HPV 16 and HPV 18 are also correlated with cervix carcinoma. Clinically the genital warts are found to grow fast and easily bleed when used physical and chemical therapy. The hyperproliferation of keratinocyte and the high lever of microvessel density with dilation of vessel lumen in derma are also confirmed by routine pathological methods. It is suggested that some factors promote cell proliferation and angiogenesis in condyloma acuminate. Our previous experiments demonstrate that VEGF, TGFβ1 are over expressed in CA. Protease-activated receptor 1 (PAR-1) is one of G Protein-coupled receptors. Its physiology functions are related to cell proliferation, angiogenesis, expressions of VEGF mRNA and so on. β -catenin is the key downstream factor of Wnt/β-catenin pathway. Wnt/β-catenin signaling transduction is a im