间歇性低压低氧对动脉粥样硬化的影响内科学专业论文.docxVIP

PAGE PAGE 3 AS 斑块不稳定性。 关键词：高海拔;间歇性低压低氧;动脉粥样硬化;斑块不稳定性 Effect of Hypobaric Hypoxia on AS in ApoE Deficient Mice Abstract Atherosclerosis is a progressive arterial disease, which is a major promoting factor of ischemic heart disease, cerebrovascular disease and peripheral vascular disease. Sudden cardiac death, one of the most common causes of death in the high altitude areas, is also considered associated with atherosclerosis. It has been reported that intermittent normobaric hypoxia experienced by patients with obstructive sleep apnea might enhance the development of atherosclerosis. Studies have shown that hypoxia may affect serum lipid profiles, attenuated endothelial function, increase level of inflammation，change plaque composition and promote the development of atherosclerosis. However, compared with the normobaric hypoxia environment at low altitudes，the effect of hypobaric hypoxia mimicking the ambient air at high altitude has not been addressed. Objective: In this study, we tested the effect of plateau intermittent hypobaric hypoxia on atherosclerosis by means of animal experiments. Methods: In this study, we used a hypobaric chamber with air pump and 20 female ApoE-deficient mice to establish a atherosclerosis model of the hypobaric hypoxia environment at high altitude.SPF ApoE-deficient mice were randomly divided into the control group and the IHH group. The two groups of mice were fed with normal diet, open eating. The control group was always in normoxic environment, while the hypoxic group was put into the low hypobaric tank which simulating the hypobaric hypoxia environment at 4000m. The hypoxic mice received hypoxia intervention for 8h/day × 8 weeks. We measured the body weight of two groups every 10 days; once every four days we recorded the food intake. Then we determined the serum lipids (TC, TG, HDL-c, LDL-c) level using commercial kits, measured the protein expression of aortic wall matrix metalloproteinases and their tissue inh