适用于棉花荧光原位杂交DNA纤维高效制备技术.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(3): 412−417 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00412 适用于棉花荧光原位杂交的DNA 纤维高效制备技术 彭仁海1,2 宋国立1 刘 方1 黎绍惠1 王春英1 张香娣1 王玉红1 王坤波1,* 1 / , 455000; 2 , 455000 : , , , DNA , Triton X-100 PVP40 , , , , , 100%, , DNA , , DNA , DNA 45S rDNA , DNA DNA , : ; ; DNA ; An Efficient Method of Cotton DNA Fibers Preparation for FISH 1,2 1 1 1 1 1 PENG Ren-Hai , SONG Guo-Li , LIU Fang , LI Shao-Hui , WANG Chun-Ying , ZHANG Xiang-Di , WANG Yu-Hong 1 and WANG Kun-Bo 1,* 1 Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture / Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 2 College of Biology and Food Technology, Anyang Institute of Technology, Anyang 455000, China Abstract: Fluorescence in situ hybridization (FISH) has become the most important technique applied in molecular cytogenetics, especially in developing physical maps in plants. As a key technique, FISH on cotton DNA