橙黄玉凤花种子萌发培养及小苗组培快繁研究.PDFVIP

2018,47(3): 273 ～276. Subtropical Plant Science 橙黄玉凤花种子萌发培养及小苗组培快繁研究 1 2 2 1 郑洲翔 ，刘德浩 ，陈智涛 ，廖建良 (1.惠州学院生命科学学院，广东 惠州 516007 ；2.惠州市林业科学研究所，广东 惠州 516001) 摘 要：对橙黄玉凤花 Habenaria rhodocheila 种子进行无菌萌发培养以获得大量原球茎，并对原球茎组培，建 立其快繁体系。结果表明，橙黄玉凤花种子以 0.1%升汞消毒 15 min 为最佳处理。种子萌发培养基中，添加 0.2% 活性碳(AC)有利于种子萌发。种子萌发的最佳培养基为 1/2MS ＋NAA 0.5 mg·L-1 ＋AC 0.2% ，原球茎增殖分化 最佳培养基为 1/2MS ＋NAA 0.2 mg·L-1 ＋ZT 3.0 mg·L-1 ＋AC 0.2% ；生根最佳培养基为 1/2MS ＋NAA 0.1 mg·L-1 ＋6-BA 2.0 mg·L-1 ＋AC 0.2% 。 关键词：橙黄玉凤花； 组织培养；快繁 Doi: 10.3969/j.issn.1009-7791.2018.03.017 中图分类号：S682.31 文献标识码：A 文章编号：1009-7791(2018)03-0273-04 Tissue Culture and Rapid Propagation of Habenaria rhodocheila 1 2 2 1 ZHENG Zhou-xiang , LIU De-hao , CHEN Zhi-tao , LIAO Jian-liang (1.School of Life Science, Huizhou University, Huizhou 516007, Guangdong China; 2.Huizhou Institute of Forestry, Huizhou 516001, Guangdong China) Abstract: Effects of different disinfection treatments, additives, plant growth regulators on tissue culture and rapid propagation system of Habenaria rhodocheila with seeds as explants were studied. Sterilizing the seeds with 0.1% mercuric chloride for 15 min was the best disinfection treatment. Seeds germinated only on the medium adding active carbon(AC). 0.2% active carbon in culture medium was fit for germination. The optimal medium for seed germination was 1/2MS ＋NAA 0.5 -1 -1 mg·L ＋AC 0.2%, the optimal medium for multiple buds