十字花科黑腐病菌中影响致病相关基因XC3814表达的基因鉴定-遗传.PDF

HEREDITAS (Beijing) 2010 1 , 32(1): 81―86 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2010.00081 十字花科黑腐病菌中影响致病相关基因XC3814 表达的 基因鉴定 1 1 1 1 1 1 1, 2 苏辉昭 , 向志娇, 彭方印 , 李瑞芳 , 安世琦 , 陆光涛, 唐纪良 1. , 530005; 2. , 530005 十字花科黑腐病菌8004 菌株的XC3814 基因与致病性和胞外多糖合成有关。文章将XC3814 的启动子与 报告基因sacB 融合, 构建了XC3814 的表达报告质粒pL3814sac 。将该质粒导入野生型菌株8004, 获得了报告 菌株8004/pL3814sac 。利用转座子EZ::Tn5 对报告菌株的基因组进行随机诱变, 分离到3 株耐蔗糖的突变体。 分析发现其中的 1 株突变体是由 EZ::Tn5 插入到编号为XC3882 的未知功能的基因所产生的。将由XC3814 启 动子与报告基因gusA 融合得到的报告质粒pGUS3814 分别导入 8004 菌株和XC3882 的转座子Tn5gusA5 插入 突变体, 测定比较pGUS3814 的GUS 表达水平, 结果显示在XC3882 突变体背景下GUS 的表达水平比在野生 型背景下降低81.3%, 表明XC3814 基因的表达水平受XC3882 基因的影响。 野油菜黄单胞菌; 致病相关基因; 表达调控 Identification of a gene involved in the expression of the pathogenicity-related gene XC3814 in Xanthomonas campestris 1 1 1 1 SU Hui-Zhao , XIANG Zhi-Jiao, PENG Fang-Yin , LI Rui-Fang , AN Shi-Qi , LU Guang-Tao1, TANG Ji-Liang1, 2 1. College of Life Science and Technology, Guangxi University, Nanning 530005, China; 2. Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530005, China Abstract: Xanthomonas campestris pv. campestris (Xcc ) is the causal agent of the black rot disease of cruciferous plants. Our previous work had demonstrated that XC3814 is required for full virulence and extracellular polysaccharide production. In this work, the reporter plasmid pL3814sac was constructed by fusing the promoter region of XC3814 to the coding region of the gene sacB, and introduced into Xcc wild-type strain 8004. The resulted strain 8004/pL3814sac was mutag