土壤中拮抗放线菌的分离和筛选-河南农业科学.PDFVIP

DOI :10.15933/j.cnki .1004 -3268.2008.01.005 2008 年第1 期 李　新,纪明山 ( , 110161) :采用稀释分离法从辽宁省不同地区采集的土样中分离到放线菌336 株。以番茄灰霉病菌 指示菌,采用平板对峙培养法筛选出抑菌效果较好的放线菌L6,O5, S2, T7,A 10 5 个菌株, 其抑菌 带宽度在 10.3 ～13.3 mm,占分离到放线菌总数的1.49%。进 一步测定5 个菌株发酵产物对番茄 灰霉病菌的抑制活性, S2 菌株的抑菌作用最强, 抑菌圈直径达23.9 mm。S2 菌株对水稻恶苗病 菌、小麦根腐病菌、烟草赤星病菌、黄瓜黑星病菌、棉花炭疽病菌、黄瓜枯萎病菌、苹果轮纹病菌、番 茄灰霉病菌、玉米小斑病菌亦有较强的抑制作用, 抑制率达55 .7%～87 .7%。 :土壤;放线菌;拮抗;植物病原真菌 :S 154.3 　　:A 　　:1004-3268(2008)0 1-0058-03 Screening of Antagonistic Actinomycetes from Soil LI Xin, I M ing-shan (Pesticide Science Lab, Plant Protection College, Shenyang Agricultural Univ ersity, Sheny ang 110161, China) Abstract:Three hundred and thirty six actinomy cetes were separated fro m soil sam ples collrcted fro m diff erent areas in Liaoning province by diluting and separating method.Using Bo try tis cine - r ea as indicator,actino mycetes strain L6,O5,S2, T7 and A 10 w as selected by plate co nfrontation method,accounting for 1.49%of the total number and w ith an inhibition zone of 10.3-13.3 mm. The inhibition eff ect of strain S2 was the best w ith an inhibitio n circle of 23.9 m m in diameter by further determining the inhibitio n effect of fermentatio n pro ducts against B ot ry t is ciner ea .The strain S2 also showed better eff ects against Gibberel la f uj ikuroi, Gibberel la f uj ikuroi,A lternar ia a lternata,Clad osp or i um cucumer in um ,Glomer ell a g ossy p i,B oty osp haeri a berengeri ana,F usari - um ox y sp orum ,B ot ry t is cinerea and B ip ol ar is may d is , w ith an inhibition rate changing from of 55.7 %to 87.7%. Key words:Soil;Actinomycetes;Inhibitio n;Plant pathogenic fung i 　　, , 8000 。 [1] ,70% 。 ,、,