响应面法优化双歧杆菌B04代谢产细菌素的发酵条件.PDFVIP

2013, Vol.34, No.03 147 B04 1 1 1 2, * 1 (1. 100048 2. 100083) (Bifi dobacterium animal)B04 B04 pH4 pH 33 1%pH 6.336 29h 3479.25AU/mL(1032.66AU/mL)2.37 Optimization of Fermentation Conditions for Bacteriocin Production from Bifi dobacterium animal B04 by Response Surface Methodology 1 1 1 2, * 1 LIU Guo-rong ZHANG Jun-ying WANG Cheng-tao LI Ping-lan REN Li (1. Beijing Key Laboratory of Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University, Beijing 100048, China 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract The fermentation conditions for bacteriocin production from Bifidobacterium animal B04 isolated from ’ centenarians intestine were optimized. The bacteriocin inhibitory activity (AU/mL) was used as evaluation index and quantified against L. monocytogenes ATCC 35152. Firstly, the effects of single factors including inoculum amount, initial pH, fermentation temperature and fermentation time on bacteriocin production from strain B04 were explored. Based on these single factor tests, response surface methodology with three variables such as initial pH, fermentation temperature and fermentation time was employed to optimize the fermentation conditions for bacteriocin production. Results showed the optimal fermentation conditions were inoculum amount of 1%, initial pH of 6.3, fermentation time of 29 h and fermentation temperature of 36 . Under these