pREP4哺乳动物表达载体说明.pdfVIP

pREP4 编号 载体名称 北京华越洋生物VECT6085 pREP4 pREP4 载体基本信息 载体名称: pREP4 Episomal Expression vector ；pREP4 质粒类型: 哺乳动物表达载体；附件体质粒 高拷贝/低拷贝: 高拷贝 克隆方法: 多克隆位点，限制性内切酶 启动子: RSV 载体大小: 10349 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: 无标签 载体抗性: 氨苄青霉素 筛选标记: 潮霉素Hygromycin 克隆菌株: TOP10 宿主细胞（系）: 人类细胞；灵长类细胞；犬类细胞 备注: pREP4 载体能够在基因组之外独立复制，是附件体型载体。 稳定性: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 pREP4 载体质粒图谱和多克隆位点信息 pREP4 载体描述 pREP4 is an episomal mammalian expression vector that uses the Rous Sarcoma Virus long terminal repeat (RSV LTR) enhancer/promoter for transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by the plasmid to permit extrachromosomal replication in human, primate and canine cells. pREP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pREP4/CAT is provided as a control for the relative level of expression of recombinant proteins in a cell line of interest. pREP4/CAT expresses the chloramphenicol acetyl transferase protein from the RSV LTR enhancer/promoter. pREP4/CAT contains the hygromycin B resistance gene for selection. pREP4 载体序列 ACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGG TCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAA TCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTATCTGAGGGGAC TAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCCTC