烟草快速繁殖及培养的研究-云南农业大学学报.PDF

13 4 Vo 1 13 No4 199 8 12 Journal of Yunnan Agricultural University Dec. 1998 * * 何川生 王晓云 雷永和 ( 653100) . : K 326G- 28 3 MS+ 6- BA 03+ IBA 005 MS + 6- BA 3+ IBA 02 35 d 50 1/ 2 MS 30 d . ; ; ; S 572 Skoog Miller ( 1957) . . . . . 1 K 326G - 28 05 % 5 min 3 MS 1 cm 1~ 2 mm . MS ( mg/ L) pH 58 24~ 28 13 h/ d 2 000~ 2 500 lx . 2 21 211 K 326G- 28 MS+ IBA 005+ 6- BA 03 30 d K 326 1998- 01- 16 * * 4 : 39 3 . G- 28 . 1 ( 30 d ) Tab . 1 Stem tips germ inating situation of diff erent variety explant K 326 G- 28 6~ 7 6 5 25 cm 24 cm 2 cm : 5 212 30 ~ 40 d . 2. MS+ IBA 005+ 6- BA 03 . 2 ( 30 d ) T ab . 2 Effect of diff erent hormone on stem tips germ ination of tobacco explant IBA 005 IAA 1 I