双黄花颗粒镇咳祛痰及免疫作用研究-天津中草药杂志社.PDFVIP

Drug Evaluation Research 第 36 卷 第 6 期 2013 年 12 月 - 431 - 双黄花颗粒镇咳祛痰及免疫作用研究 1 1 2 2 2 2 * 刘 林 ，杨 蕙 ，廖 君 ，易亚乔 ，彭买姣 ，王宇红 1. 湖南中医药大学第一附属医院，湖南 长沙 410007 2. 湖南中医药大学，湖南 长沙 410208 摘 要：目的 观察双黄花颗粒的镇咳、祛痰及调节免疫作用。方法 通过小鼠氨水引咳和豚鼠枸橼酸引咳试验，观察双黄 花的镇咳作用；通过小鼠酚红排泄试验观察双黄花的祛痰作用；通过小鼠碳粒廓清试验，观察双黄花的调节免疫作用。 结果 在小鼠氨水引咳试验中，双黄花高、中剂量组能显著延长浓氨水致咳的潜伏期，差异具有统计学意义（P ＜0.05 ）；双 黄花高剂量组能显著减少小鼠浓氨水致咳的次数，差异具有统计学意义（P ＜0.05 ）；在豚鼠枸橼酸引咳试验中，双黄花高剂 量组能显著延长豚鼠枸橼酸引咳的潜伏期和减少咳嗽次数，差异具有统计学意义（P ＜0.01 ）；在小鼠酚红排泄试验中，双黄 花高剂量组能显著增加气管酚红排泄量，差异具有统计学意义（P ＜0.05 ）；在小鼠碳粒廓清试验中，双黄花高、中剂量组显 著提高小鼠廓清指数和吞噬指数，差异具有统计学意义（P ＜0.05、0.01 ）。结论 双黄花颗粒具有较好的镇咳、祛痰及调节 免疫作用。 关键词：双黄花颗粒；急性上呼吸道感染；镇咳；祛痰；调节免疫 中图分类号：R974 文献标志码：A 文章编号：1674 - 6376 (2013) 06 - 0431 - 04 DOI: 10.7501/j.issn.1674-6376.2013.06.007 Study on relieving cough, expelling phlegm, and immunomodulation of Shuanghuanghua Granule 1 1 2 2 2 2 LIU Lin , YANG Hui , LIAO Jun , YI Ya-qiao , PENG Mai-jiao , WANG Yu-hong 1. First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China 2. Hunan University of Chinese Medicine, Changsha 410208, China Abstract: Objective To evaluate the relieving cough, expelling phlegm, and immunomodulation of Shuanghuanghua Granule (SG). Methods Cough was induced by ammonia in mice and citric acid in guinea pig. The number of cough was recorded to indicate the effect of SG. The expectorant activities were detected by testing the amount of phlegm secreted in mice. Immunomodulation was evaluated by testing the expectorant carbon clearance in mice. Results Cough was induced by ammonia in mice, high- and mid-dose SG groups could apparently prolong the coughing incubation period (P 0.05) and high-dose SG group could apparently reduce coughing frequency (P 0.05); Cough was induced by