探针应用概要STED（StimulatedEmissionDepletionfluorescence.pptVIP

动态成像 · 囊泡运输 · 细胞膜脂筏 · 等等 Mitochondria （线粒体） Figure 1. Simulations. (a) Point spread functions (PSFs) drawn to scale of (i) a confocal microscope (diffraction limited), (ii) a super-resolution (SR) microscope enabling a resolution of 30 nm in the optical plane and conventional resolution along the z-axis, and (iii) a super-resolution microscope enabling isotropic resolution of 30 nm. This resolution was chosen because it can be attained with various instruments and because antibody labeling using a primary and a dye labeled secondary antibody would increase any structure to about this siz