利用Cytb基因由未知鲸骨鉴定出大村鲸.PDF

兽类学报,2007,27 (3):288 - 292 Acta Theriologica Sinica 利用 Cyt b 基因由未知鲸骨鉴定出大村鲸  马牧 祝茜 李响 孙玉苗 刘莹莹 刘亚楠 陶翠花 (山东大学威海分校海洋学院, 威海 264209 ) 关键词:鲸类脊椎骨;细胞色素b 基因;分子进化树;种类鉴定;大村鲸 中图分类号:Q754 文献标识码:A 文章编号:1000 - 1050 (2007 )03 - 0288 - 05 Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence  MA Mu,ZHU Qian ,LI Xiang,SUN Yumiao,LIU Yingying,LIU Yanan,TAO Cuihua (Ocean College,Shandong University at Weihai ,Weihai 264209 , China) Abstract :In order to identify an unknown whale vertebra ’s species,genomic DNA is extracted from the bone powder by proteinase K and phenol/ chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR (Polymerase Chain Reaction). The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai,it is 99%  It indicates that the vertebra is Balaenoptera omurai The phylogenetic tree based on UPGMA is consist- ent with the blasting result Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1 - 2 nucleotides which are different from the homologous sequence of B omurai (accession AB201257,AB201256 ),thus, the sequence is a new haplotype This is the first record of B omurai which distributes in the South China Sea. Key words :Balaenoptera omurai;Cytochrome b gene ;Phylogenetic tree ;Species identification ;Whale vertebra 作者于2005 年2 月从广东省湛江市硇洲岛一 行比对,并辅以生物学软件分析,以期对该脊椎骨 位渔民手中采集到一块鲸类的脊椎骨,据渔民对该 的种类进行分子生物学的初步鉴定。 搁浅鲸类的外形描述推断该骨骼属于一种大型须 1 材料与方法 鲸。为了鉴定该脊椎骨所属的种类,本文利用分子 生物学技术,从中提取基因组 DNA,并以此为模 1 1 样品 板特异性扩增 Cyt b 基