不同起源胶质瘤中microRNA-10表达差异及其和星形胶质细.docVIP

不同起源胶质瘤中microRNA-210表达差异及其和星形胶质细胞肿瘤级别相关性的研究 赖年升1 张帅1 成宜军2 徐幸1 蔺玉昌1 1.南京医科大学附属无锡第二人民医院神经外科 江苏 无锡 214000 2,南京医科大学附属脑科医院 江苏 南京 210029 【摘要】 目的 探讨microRNA-210（miR-210）在星形胶质细胞肿瘤和少突胶质细胞肿瘤中的表达差异及意义。方法 将组织标本分为正常脑组织、少突胶质细胞肿瘤、星形胶质细胞肿瘤三组，星形胶质细胞肿瘤再分为A[毛细胞型星形细胞瘤（WHO Ⅰ级）]，B[弥漫性星形细胞瘤（WHO Ⅱ级）]，C[间变性星形细胞瘤（WHO Ⅲ级）]，D[胶质母细胞瘤（GBM,WHO Ⅳ级）]。采用实时定量PCR方法检测各组中miR-210的表达水平。 统计学分析miR-210的表达和星形胶质细胞肿瘤的相关性。结果 miR-210在各级别星形胶质细胞肿瘤中表达水平依次为D,C,(B,A)(P0．001)，除A、B组间外，其他组间都具有统计学意义(P0．001)，说明星形胶质细胞肿瘤级别越高，miR-210表达水平越高；然而在少突胶质细胞肿瘤中呈低表达(P0．05),且间变性少突胶质细胞瘤中表达量低于少突胶质细胞瘤中的表达量。结论 miR-210在不同起源、不同级别胶质瘤中的表达水平不同，可以作为不同起源胶质瘤病理检查的辅助鉴别手段，也可作为星形胶质细胞肿瘤恶性进展的生物学标志物。miR-210的表达水平与星形胶质细胞肿瘤和少突胶质细胞肿瘤病理分级具有相关性，miR-210的表达量随着星形细胞肿瘤级别的增高而增加，随着少突胶质细胞肿瘤级别的增高而降低，提示miR-210与胶质瘤的预后密切相关。miR-210表达水平在二种不同起源的胶质瘤中表达具有差异，在少突胶质细胞肿瘤中表达降低，在星形胶质细胞肿瘤中表达增高。 【关键词】 胶质瘤；星形胶质细胞肿瘤；少突胶质细胞肿瘤；MicroRNA-210；预后 Differential expression of microRNA-210 in gliomas of variable cell origin and correlation of increased expression levels to disease grade in astrocytic tumors LAI Nian-sheng*1,ZHANG Shuai1, CHENG Yi-jun2, XU Xing, LIN Yu-chang*1 Department of Neurosurgery, Wuxi Second Peoples Hospital Af?liated to Nanjing Medical University, Wuxi City 214002, China Nanjing Brain Hospital Affiliated toNanjing Medicial University，Nanjing 210029，China Corresponding author:LIN Yu-chang,Email:yuchanglin123@126.com 【Abstract】 Objective To investigate the significance and expression of microRNA-210 in astrocytic tumors and oligodendroglial tumors.Methods Tissue samples were divided into three groups of the normal brain tissue，the oligodendroglial tumor and the astrocytic tumor; The astrocytic tumors were divided into three groups of A[pilocytic astrocytoma (WHO grade I)]，B[diffuse astrocytomas (WHO grade II)], C[anaplastic astrocytomas(WHO grade III)], and D[glioblastoma (GBM, WHO grade IV)]. The expression of miR-210 was examined by real-time quantitative RT-PCR. The correlation of the expression of miR-210 and the astrocytic tumors were analyzed by the Spearm