烟酸羟基化菌株睾酮丛毛单胞菌JA1抗性质粒的消除-南京师范大学学报.PDF

30 2 () Vol. 30 No. 2 2007 6 JOURNAL OF NANJING NORMAL UNIVERSITY (Natural Science Edition) Jun, 2007 烟酸羟基化菌株睾酮丛毛单胞菌 JA1抗性质粒的消除 杨 瑶, 袁 生, 尚广东,戴亦军 (, , 210097) [] JA16- , . JA1, 19 kb. 0002 5% SDS, , , , . , , JA1, . [] , , ComamonastestosteroniJA1, [] 819 [] A [] 2007) Elimination of theResistance Plasmidin Niconic Acid Hydroxylation Strain Comamonastestosteroni JA1 Yang Y ao, Y uan Sheng, Shang G uangdong, Da i Y ijun ( Jiangsu Key Laboratory of Biodiversity and Biotechnology, Key Lab ofM icrobialTechnology in the School of Life Science, N anjingNormalUniversity, Nanjing 210097, China) Abstract:Comamonastestosteroni JA1, catalyzing the hydroxylation from nicotinic acid to 6- hydroxynico tinicacid, was found to resist to many antibiotics. A lkaline lysis extraction of the JA1 culture reveals a 19 kb plasm id. 0002 5% SDS treatment of the strain elmi inated the extrachromosal plasm id. The mutants lost the kanamycin resistance ability while keeping the nicotinic acid hydroxylation activity. K ey words: plasm id elmi ination, nicotinic acid dehydrogenase, Comamonastestosteroni JA 1, antibiotic resistance 0 引言 [ 1- 3] 6- ,, [4- 8] 6- . JA 1 6- , . [9] 6- , . , ,, 6- ., [ 10- 12] , ,. JA1, ., JA 1 ,,, . [ 13- 17] ., , : : : ( 06KJA21016) (06- C- 014). : ( 1981! ), , , . Email: cranny yang@ hotmail. com